- International Journal of Systematic and Evolutionary Microbiology
- Volume 60, Issue 5
f Natronomonas moolapensis sp. nov., non-alkaliphilic isolates recovered from a solar saltern crystallizer pond, and emended description of the genus Natronomonas
- Authors: David G. Burns1, Peter H. Janssen2, Takashi Itoh3, Hiroaki Minegishi4, Ron Usami4, Masahiro Kamekura5, Mike L. Dyall-Smith6
- VIEW AFFILIATIONS
Affiliations: 1 1Microbiological Diagnostic Unit, University of Melbourne, Parkville 3052, Australia 2 2Grasslands Research Centre, Tennent Drive, Private Bag 11008, Palmerston North 4442, New Zealand 3 3Japan Collection of Microorganisms, RIKEN BioResource Center, Saitama 351-0198, Japan 4 4Bio-Nano Electronics Research Centre, Toyo University, Kawagoe 350-8585, Japan 5 5Halophiles Research Institute, 677-1 Shimizu, Noda-shi, Chiba 278-0043, Japan 6 6Max Planck Institute of Biochemistry, Department of Membrane Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany
- CorrespondenceMike L. Dyall-Smith firstname.lastname@example.org
- Int J Syst Evol Microbiol, May 2010 60: 1173-1176, doi: 10.1099/ijs.0.010132-0
- Subject: New Taxa - Archaea
- Published Online:
Two isolates of non-alkaliphilic, extremely halophilic archaea, with very similar characteristics, were recovered from a marine solar saltern crystallizer. Cells were pleomorphic, motile and Gram-stain-negative and grew on a limited range of carbon sources, with pyruvate being the best substrate. Optimum growth occurred at 18–20 % (w/v) NaCl, pH 6.0–8.5 and 37–40 °C. Both isolates possessed typical archaeal lipids, and their 16S rRNA gene sequences were 99.8 % identical. Phylogenetic tree reconstructions indicated that they were most closely related to the haloalkaliphile Natronomonas pharaonis (97.5 % similarity to the type strain), but the different phenotypic properties and low DNA–DNA hybridization values between Nmn. pharaonis DSM 2160T and the two isolates suggested that they represent a novel species within the genus Natronomonas. The name Natronomonas moolapensis sp. nov. is proposed for these isolates, with the type strain being 8.8.11T (=JCM 14361T =CECT 7526T =DSM 18674T). An emended description of the genus Natronomonas is also provided.
The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of isolates 8.8.11T and 4.03.5 are AY498645 and EF127644, respectively.
SGM | Published by the International Union of Microbiological Societies
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