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Halobacillus salsuginis sp. nov., a moderately halophilic bacterium from a subterranean brine

¹ Jishou University;
² Chinese Academy of Medical Sciences and Peking Union Medical College;
³ Deutsche Sammlung von Mikroorganismen und Zellkulturen;
⁴ Yunnan Institute of Microbiology, Yunnan University

^* Corresponding author; email: wjli{at}ynu.edu.cn

A moderately halophilic, endospore-forming, Gram-positive, catalase- and oxidase-positive, motile, rod-shaped, aerobic bacterium, strain JSM 078133T, was isolated from a subterranean brine sample collected from a salt mine in Hunan province, China. Strain JSM 078133T was able to grow with 1–23 % (w/v) total salts (optimum 5–10 %) and at pH 6.0–10.0 (optimum pH 7.5) and 10–45 °C (optimum 30 °C). meso-Diaminopimelic acid was present in the cell-wall peptidoglycan. The predominant respiratory quinone was menaquinone 7 (MK-7) and the major cellular fatty acids were anteiso-C15:0, anteiso-C17:0 and iso-C15:0. The genomic DNA G + C content was 42.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain JSM 078133T belongs to the genus Halobacillus, being related most closely to the type strain of Halobacillus campisalis (sequence similarity 97.1 %), followed by Halobacillus alkaliphilus (97.0 %) and Halobacillus yeomjeoni (96.8 %). The combination of phylogenetic analysis, DNA–DNA relatedness, phenotypic characteristics and chemotaxonomic data supported the view that strain JSM 078133T represents a new species of the genus Halobacillus, for which the name Halobacillus salsuginis sp. nov. is proposed, with JSM 078133T (=DSM 21185T =KCTC 13236T) as the type strain.