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This Article Full Text (Papers in Press[PDF]) All Versions of this Article: ijs.0.007674-0v1 59/7/1748    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Google Scholar Articles by Sakamoto, M. Articles by Benno, Y. PubMed PubMed Citation Articles by Sakamoto, M. Articles by Benno, Y. Agricola Articles by Sakamoto, M. Articles by Benno, Y.

Butyricimonas synergistica gen. nov., sp. nov. and Butyricimonas virosa sp. nov., butyric acid-producing bacteria in the family Porphyromonadaceae isolated from rat faeces

¹ Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center;
² Laboratory of Tea Function R&D, Food Research Laboratories, Mitsui Norin Co. Ltd;
³ Laboratory of Quality Control R&D, Food Research Laboratories, Mitsui Norin Co. Ltd

⁴ E-mail: sakamoto{at}jcm.riken.jp

Two bacterial strains isolated from rat faeces, MT01T and MT12T, were characterized using a polyphasic taxonomic approach that included analysis of the phenotypic and biochemical features, cellular fatty acid profiles, menaquinone profiles, and phylogenetic position based on 16S rRNA gene sequence analysis. The 16S rRNA gene sequence analysis showed that these strains were members of the family Porphyromonadaceae. These strains shared 94 % 16S rRNA gene sequence similarity with each other and were related to Odoribacter splanchnicus with 86-87 % sequence similarity. The strains were obligately anaerobic, non-pigmenting, non-sporeforming, non-motile, Gram-negative rods. The growth of the strains was inhibited on medium containing 20 % bile. The two strains produce significant levels of butyric and isobutyric acids as the end-products from glucose. Although the major cellular fatty acid of these two strains and O. splanchnicus JCM 15291T was iso-C15:0, strains MT01T and MT12T showed higher level of iso-C15:0 (66 % and 74 %, respectively) than O. splanchnicus JCM 15291T (48 %). In addition, the ratios of iso-C15:0 to anteiso-C15:0 in whole-cell methanolysates of two isolates were very much higher than that of O. splanchnicus JCM 15291T. The major menaquinone of the isolates was MK-10. This menaquinone composition was different from those of other genera of the family Porphyromonadaceae, such as Barnesiella (predominant menaquinones: MK-11 and MK-12), Odoribacter (MK-9), Paludibacter (MK-8), Parabacteroides (MK-9 and MK-10), Porphyromonas (MK-9 and MK-10), Tannerella (MK-10 and MK-11). These are important chemotaxonomic characteristics of these microorganisms. The G+C content of these strains is 46 mol%. On the basis of these data, It is proposed that these strains should be classified in a novel genus Butyricimonas, as Butyricimonas synergistica gen. nov., sp. nov., which is also the type species and Butyricimonas virosa sp. nov. The type strains of B. synergistica and B. virosa are MT01T ( = JCM 15148T = CCUG 56610T) and MT12T ( = JCM 15149T = CCUG 56611T), respectively.