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Sediminimonas qiaohouensis gen. nov., sp. nov., a novel member of the Roseobacter clade in the order Rhodobacterales

¹ Yunnan Institution of Microbiology;
² College of Bio-resources and Environmental Science, Jishou University, Jishou, Hunan 416000, P. R. C;
³ Agricultural Cultural Collection of China, Institute of Agricultural Resources and Regional Planning

⁴ E-mail: xlcuiynu{at}yahoo.com.cn

Two aerobic bacterial strains, YIM B024T and YIM B025, were isolated from a salt mine in Yunnan, south-west China. Both strains showed almost the same physiological properties. Cells were Gram-negative, non-motile, non-sporeforming rods. The strains grew at 15–37 °C, pH 6.5–.0 and 0.25–20 % NaCl; optimum growth was observed at 28-30 °C, pH 7.0-8.5 and 1.5-10 % NaCl. Oxidase, catalase and nitrate-reducing activities were detected. The two strains were closely related to each other with 16S rRNA gene sequences similarity of 100 %. DNA-DNA hybridization experiments revealed high values (90±0.4 %) for relatedness between strains YIM B024T and YIM B025, which suggested that these two strains constituted a single species. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the two isolates formed a loosely cluster with members of the genus Roseivivax in the Roseobacter clade, but were clearly separated from the genus. The levels of 16S rRNA gene sequences similarity between the two isolates and members of the genus Roseivivax ranged from 92.4 to 93.9 %. The major polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and four unknown phospholipids. The major cellular fatty acids were C18:17c, C16:0, C18:19c, 11-methyl C18:17c and C19:0 cyclo 8c. Q-10 was the sole respiratory quinone and the genomic DNA G+C content was 63.0-64.1 mol%. The distinct phylogenetic position and combination of phenotypic and chemotaxonomic characteristics supported the proposal of the isolates representing a novel species in a new genus, for which the name Sediminimonas qiaohouensis gen. nov., sp. nov. is proposed, with the type strain YIM B024T (= KCTC 22346T= CCTCC AA208033T).