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Sphaerisporangium flaviroseum sp. nov. and Sphaerisporangium album sp. nov., isolated from forest soil in China

Yunnan Institut of Microbiology

¹ E-mail: lihxu{at}ynu.edu.com

Two Gram-positive, aerobic actinomycete strains YIM 48771^T and YIM 48782^T were isolated from virgin forest soil samples collected in Hunan Province, China. The two strains belong to the genus Sphaerisporangium according to a polyphasic taxonomic approach. Based on the phylogenetic analysis, similarities of 16S rRNA gene sequence of YIM 48771^T with four valid published species, Sphaerisporangium viridialbum, Sphaerisporangium cinnabarinum, Sphaerisporangium melleum, Sphaerisporangium rubeum and YIM 48782^T were respectively 97.3%, 97.4%, 97.2%, 96.3% and 97.1%, and those of YIM 48782^T with the four type species were 96.9%, 97.6%, 96.9% and 97.1%. Results from DNA-DNA hybridization of the two strains with the four species revealed that DNA homologies of them were below 64.1%. MK-9 of the two strains was 28.1% and 29.0%, but those of the four type species are small amount. Contents of fatty acid C15:0, iso-C15:0 and C17:0 of the two strains were below 10%, and no C17:18c, but S. viridialbum and S. rubeum are more than 10%. C16:0 of YIM 48771^T was 15.02%, but the four species and YIM 48782^T are below 10.3%. The two strains contented diphosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylethanolamine and phosphoglycolipids, which are identical with the genus. Besides, strain YIM 48771^T contented phosphatidylmethylethanolamine and phosphatidylinositol, and strain YIM 48782^T phosphatidylinositol. G+C of YIM 48771^T was 67.1%, and below those of the four species and YIM 48782^T. So we propose two novel species, Sphaerisporangium flavoroseum YIM 48771^T (=DSM 45170^T =KCTC 19393^T) and Sphaerisporangium album YIM 48782^T (=DSM 45172^T =CCTCC AA 208026^T).