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Published online ahead of print on 19 June 2009 as doi:ijs.0.005967-0
International Journal of Systematic and Evolutionary Microbiology 2009;59:1650.

Int J Syst Evol Microbiol (2009); DOI 10.1099/ijs.0.005967-0
© 2009 International Union of Microbiological Societies
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Enterobacter oryzae sp. nov., a nitrogen-fixing bacterium isolated from wild rice Oryza latifolia

Guixiang Peng1, Wu Zhang2, huifen Luo2, Hongwei Xie1, Weihao Lai2 and Zhiyuan Tan3,4

1 College of Resources and Environment, South China Agricultural University, Guangzhou 510642, China;
2 Provincial Key Lab of Plant Molecular Breeding, College of Agriculture, South China Agricultural Uni;
3 South China Agricultural University

4 E-mail: zytan{at}scau.edu.cn

Twelve facultatively anaerobic, endophytic diazotrophs were isolated from surface sterilized roots of wild rice Oryza latifolia and clustered by phenotypic and molecular methods. Six isolates were grouped together as Group A by phenotypic characters, and confirmed by SDS-PAGE whole-cell protein patterns and insertion sequence-based polymerase chain reaction (IS-PCR) methods. Phylogenetic analysis derived from the 16S rRNA gene sequence indicated that Group A represented by strain Ola 51T is closely related to Enterobacter radicincitans (98.9 % similarity, except Enterobacter radicincitans DSM 16656T has 70 bp insertion) and Enterobacter cloacae (98.0 % similarity). The rpoB gene sequence analysis also showed strain Ola 51T has the highest sequence similarities to Enterobacter radicincitans DSM 16656T (98.3%), but supported the distinct position. Biological and biochemical tests, protein patterns, genomic DNA finger-printing, antibiotic resistance and comparison of cellular fatty acids showed the difference among Group A, Enterobacter radicincitans DSM 16656T and Enterobacter cloacae ATCC 13047T. DNA-DNA hybridization distinguished strain Ola 51T from the closely related Enterobacter species phylogenetically. Based on these data, a novel species, Enterobacter oryzae sp. nov. is proposed, with strain Ola 51T (=LMG 24251T=CGMCC 1.7012T) as the type strain.






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