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1 National Institute of Agricultural Science and Technology;
2 Korean Agricultural Culture Collection (KACC)
3 E-mail: swkwon{at}rda.go.kr
Two novel strains, designated JS12-10T and JS14-6T, isolated from Jeju soils in South Korea, were characterized using a polyphasic approach to clarify their taxonomic positions. These isolates were found to be aerobic, Gram-negative, motile with one polar flagellum, non-spore-forming and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these two isolates formed a cluster with Dyella koreensis BB4T, Dyella japonica XD53T and Frateuria aurantia DSM 6220T. The sequence similarity between JS12-10T and JS14-6T was 97.1 %. Strain JS12-10T showed the highest sequence similarity with Dyella koreensis BB4T (98.1 %), and strain JS14-6T showed the closest relationships with Dyella japonica XD53T (98.2 %). Phenotypic and fatty acid data supported the affiliation of these strains to the genus Dyella. However, the results of physiological and biochemical tests allowed phenotypic differentiation of the isolates from Dyella species with validly published names. Therefore, strains JS12-10T and JS14-6T represent two novel species of the genus Dyella, for which the names Dyella soli sp. nov. (type strain JS12-10T =KACC KACC 12747T =JCM 15423T), and Dyella terrae sp. nov. (type strain JS14-6T = KACC 12748T =JCM 15424T) are proposed.
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