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This Article Full Text (Papers in Press[PDF]) All Versions of this Article: ijs.0.003087-0v1 59/7/1805    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Google Scholar Articles by Clavel, T. Articles by Haller, D. PubMed PubMed Citation Articles by Clavel, T. Articles by Haller, D. Agricola Articles by Clavel, T. Articles by Haller, D.

Isolation of bacteria from the ileal mucosa of TNF^deltaARE mice and description of Enterorhabdus mucosicola gen. nov., sp. nov.

¹ Technische Universität München;
² Novabiotics Ltd.;
³ DIfE;
⁴ TUM

⁵ E-mail: thomas.clavel{at}wzw.tum.de

The diversity of bacteria associated with inflamed mucosa was investigated by culturing ileal samples from TNFdeltaARE mice on a selective medium containing mucin. Among eight isolates, two strains (Mt1B3 and Mt1B8) belonged to bacterial groups not yet cultured from the mouse intestine. Whereas strain Mt1B3 was identified as a member of the family Planococcaceae and is closely related to Sporosarcina spp. and Filibacter limicola, strain Mt1B8 was a novel bacterium. Based on phylogenetic analysis, strain Mt1B8 is a member of the family Coriobacteriaceae. The closest related described bacteria were Asaccharobacter celatus and Adlercreutzia equolifaciens (< 96 % similarity) and Eggerthella spp. (< 92 %). With respect to As. celatus and Eggerthella spp., the phylogenetic position of strain Mt1B8 was confirmed at the chemotaxonomic level by FT-IR spectroscopic analysis. The major fatty acid of strain Mt1B8 is C16:0 (23.9 %). Menaquinones were monomethylated. DNA-DNA relatedness between strain Mt1B8 and As. celatus was 28 %. Strain Mt1B8 is a Gram-positive rod that does not form spores and has a high G + C content of DNA (64.2 mol%). Cells are aerotolerant but grow only under strictly anoxic conditions. They are sensitive to cefotaxime, clarithromycin, erythromycin, metronidazole, tetracycline, tobramycin and vancomycin. API and Vitek analysis showed the ability of strain Mt1B8 to convert a variety of amino acid derivatives. According to these findings, it is proposed to create a novel genus, Enterorhabdus, and a novel species, Enterorhabdus mucosicola, to accommodate strain Mt1B8. The type strain is Mt1B8T (= DSM 19490T = CCUG 54980T).