HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (Papers in Press[PDF]) All Versions of this Article: ijs.0.002469-0v1 59/7/1635    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Google Scholar Articles by Chen, Y.-G. Articles by Li, W.-J. PubMed PubMed Citation Articles by Chen, Y.-G. Articles by Li, W.-J. Agricola Articles by Chen, Y.-G. Articles by Li, W.-J.

Pontibacillus halophilus sp. nov., a moderately halophilic bacterium isolated from sea urchin

¹ Jishou University;
² Chinese Academy of Medical Sciences& Peking Union Medical College;
³ Yunnan University

⁴ E-mail: wjli{at}ynu.edu.cn

A Gram-positive, moderately halophilic, strictly aerobic, catalase- and oxidase-positive, rod-shaped bacterium, strain JSM 076056T, was isolated from a sea urchin collected from the South China Sea. Cells were motile by means of peritrichous flagella and formed ellipsoidal endospores lying in subterminal swollen sporangia. Strain JSM 076056T was able to grow at salinities of 2-25 % (w/v) total salts and at pH 6.0-10.0 and 15-40 °C; optimum growth was observed with 5-10 % (w/v) total salts and at pH 7.0-8.0 and 25-30 °C. It was incapable of growing with NaCl as sole salts. meso-Diaminopimelic acid was present in the cell-wall peptidoglycan. Iso-C15:0, iso-C16:0, iso-C14:0, anteiso-C15:0 and C16:17c alcohol were the major cellular fatty acids. The predominant respiratory quinone was menaquinone 7 (MK-7) and the genomic DNA G + C content was 45.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JSM 076056T belonged to the family Bacillaceae and was related most closely to the type strains of the two recognized species of the genus Pontibacillus, Pontibacillus chungwhensis BH030062T (sequence similarity 96.4 %) and Pontibacillus marinus BH030004T (96.2 %), and these three strains formed a robust cluster in the phylogenetic tree. The combination of phylogenetic, phenotypic and chemotaxonomic data supported that strain JSM 076056T represents a novel species of the genus Pontibacillus, for which the name Pontibacillus halophilus sp. nov. is proposed. The type strain is JSM 076056T (=CCTCC AA 207029T =DSM 19796T =KCTC 13190T).

This article has been cited by other articles:

				Y.-G. Chen, Y.-X. Wang, Y.-Q. Zhang, S.-K. Tang, Z.-X. Liu, H.-D. Xiao, L.-H. Xu, X.-L. Cui, and W.-J. Li Nocardiopsis litoralis sp. nov., a halophilic marine actinomycete isolated from a sea anemone Int J Syst Evol Microbiol, November 1, 2009; 59(11): 2708 - 2713. [Abstract] [Full Text] [PDF]

				Y.-G. Chen, Y.-Q. Zhang, H.-Y. Huang, H.-P. Klenk, S.-K. Tang, K. Huang, Q.-H. Chen, X.-L. Cui, and W.-J. Li Halomonas zhanjiangensis sp. nov., a halophilic bacterium isolated from a sea urchin Int J Syst Evol Microbiol, November 1, 2009; 59(11): 2888 - 2893. [Abstract] [Full Text] [PDF]