HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary Figure Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Google Scholar Articles by Hetzer, A. Articles by Morgan, H. W. PubMed PubMed Citation Articles by Hetzer, A. Articles by Morgan, H. W. Agricola Articles by Hetzer, A. Articles by Morgan, H. W.

Venenivibrio stagnispumantis gen. nov., sp. nov., a thermophilic hydrogen-oxidizing bacterium isolated from Champagne Pool, Waiotapu, New Zealand

¹ Thermophile Research Unit, University of Waikato, Te Whare Wananga o Waikato, Private Bag 3105, Hamilton 3240, New Zealand
² Department of Biological Sciences, University of Waikato, Te Whare Wananga o Waikato, Private Bag 3105, Hamilton 3240, New Zealand

Correspondence
Adrian Hetzer
hetzer.adrian{at}web.de

A novel thermophilic, hydrogen-oxidizing bacterium, designated strain CP.B2^T, was isolated from a terrestrial hot spring in Waiotapu, New Zealand. Cells were motile, slightly rod-shaped, non-spore-forming and Gram-negative. Isolate CP.B2^T was an obligate chemolithotroph, growing by utilizing H₂ as electron donor and O₂ as corresponding electron acceptor. Elemental sulfur (S⁰) or thiosulfate () was essential for growth. Microbial growth occurred under microaerophilic conditions in 1.0–10.0 % (v/v) O₂ [optimum 4–8 % (v/v) O₂], between 45 and 75 °C (optimum 70 °C) and at pH values of 4.8–5.8 (optimum pH 5.4). The DNA G+C content was 29.3 mol%. 16S rRNA gene sequence analysis demonstrated that strain CP.B2^T belonged to the order Aquificales, with a close phylogenetic relationship to Sulfurihydrogenibium azorense (94 % sequence similarity to the type strain). However, genotypic and metabolic characteristics differentiated the novel isolate from previously described genera of the Aquificales. Therefore, CP.B2^T represents a novel species in a new genus, for which the name Venenivibrio stagnispumantis gen. nov., sp. nov. is proposed. The type strain of Venenivibrio stagnispumantis is CP.B2^T (=JCM 14244^T =DSM 18763^T).

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA and polA gene sequences of strain CP.B2^T are DQ989208 and EF581124.

A graph showing the effect of temperature on growth of cells of strain CP.B2^T is available as supplementary material with the online version of this paper.