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Methylobacterium oryzae sp. nov., an aerobic, pink-pigmented, facultatively methylotrophic, 1-aminocyclopropane-1-carboxylate deaminase-producing bacterium isolated from rice

¹ Department of Agricultural Chemistry, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea
² Microbial Genetics Division, National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon 441-707, Republic of Korea

Correspondence
Tong-Min Sa
tomsa{at}chungbuk.ac.kr

A pink-pigmented, facultatively methylotrophic bacterium, strain CBMB20^T, isolated from stem tissues of rice, was analysed by a polyphasic approach. Strain CBMB20^T utilized 1-aminocyclopropane 1-carboxylate (ACC) as a nitrogen source and produced ACC deaminase. It was related phylogenetically to members of the genus Methylobacterium. 16S rRNA gene sequence analysis indicated that strain CBMB20^T was most closely related to Methylobacterium fujisawaense, Methylobacterium radiotolerans and Methylobacterium mesophilicum; however, DNA–DNA hybridization values were less than 70 % with the type strains of these species. The DNA G+C content of strain CBMB20^T was 70.6 mol%. The study presents a detailed phenotypic characterization of strain CBMB20^T that allows its differentiation from other Methylobacterium species. In addition, strain CBMB20^T is the only known member of the genus Methylobacterium to be described from the phyllosphere of rice. Based on the data presented, strain CBMB20^T represents a novel species in the genus Methylobacterium, for which the name Methylobacterium oryzae sp. nov. is proposed, with strain CBMB20^T (=DSM 18207^T=LMG 23582^T=KACC 11585^T) as the type strain.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain CBMB20^T is AY683045.

Photomicrographs of strain CBMB20^T, 16S rRNA gene sequence similarity data and biochemical reactions that differentiate the novel strains from related species are available as supplementary material in IJSEM Online.

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