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Lipomyces orientalis sp. nov., a yeast species isolated from soil in Vietnam

Department of Microbiology, Food Industries Research Institute, 301-Nguyen Trai, Thanh Xuan, Hanoi, Vietnam

Correspondence
Vu Nguyen Thanh
thanh{at}firi.ac.vn

Five strains of the novel ascogenous yeast species Lipomyces orientalis sp. nov. were isolated from soil samples collected in Vietnam. The yeast forms asci containing one to four ascospores. The ascospores of L. orientalis have warty surfaces and differ from the characteristic striated ascospores of Lipomyces tetrasporus. Phylogenetic analyses of D1/D2 26S rDNA and ITS sequences indicate that L. orientalis and L. tetrasporus are closely related, but they differ from each other by 6 nucleotides in the D1/D2 region and 16 nucleotides in the ITS region. The type culture is strain Lip 95^T (=CBS 10300^T=NRRL Y-27927^T).

A protocol for the preparation of malt broth, a map showing the locations of the sampling sites, an electron micrograph of ascospores of strain Lip 95^T and a neighbour-joining tree based on D1/D2 sequences are available as supplementary material in IJSEM Online.