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Int J Syst Evol Microbiol 56 (2006), 2887-2892; DOI  10.1099/ijs.0.64450-0
© 2006 International Union of Microbiological Societies

Rhodonellum psychrophilum gen. nov., sp. nov., a novel psychrophilic and alkaliphilic bacterium of the phylum Bacteroidetes isolated from Greenland

Mariane Schmidt1,2,{dagger}, Anders Priemé2 and Peter Stougaard1,3

1 Bioneer A/S, Kogle Allé 2, Hørsholm, Denmark
2 Department of Biology, University of Copenhagen, Sølvgade 83H, Copenhagen, Denmark
3 Department of Ecology, Royal Veterinary and Agricultural University, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark

Correspondence
Peter Stougaard
psg{at}kvl.dk

A novel alkaliphilic and psychrophilic bacterium was isolated from the cold and alkaline ikaite tufa columns of the Ikka Fjord in south-west Greenland. According to 16S rRNA gene sequence analysis, strain GCM71T belonged to the family ‘Flexibacteraceae’ in the phylum Bacteroidetes. Strain GCM71T, together with five related isolates from ikaite columns, formed a separate cluster with 86–93 % gene sequence similarity to their closest relative, Belliella baltica. The G+C content of the DNA from strain GCM71T was 43.1 mol%, whereas that of B. baltica was reported to be 35 mol%. DNA–DNA hybridization between strain GCM71T and B. baltica was 9.5 %. The strain was red pigmented, Gram-negative, strictly aerobic with non-motile, rod-shaped cells. The optimal growth conditions for strain GCM71T were pH 9.2–10.0, 5 °C and 0.6 % NaCl. The fatty acid profile of the novel strain was dominated by branched and unsaturated fatty acids (90–97 %), with a high abundance of iso-C17 : 1{omega}9c (17.5 %), iso-C17 : 0 3-OH (17.5 %) and summed feature 3, comprising iso-C15 : 0 2-OH and/or C16 : 1{omega}7c (12.6 %). Phylogenetic, chemotaxonomic and physiological characteristics showed that the novel strain could not be affiliated to any known genus. A new genus, Rhodonellum gen. nov., is proposed to accommodate the novel strain. Strain GCM71T (=DSM 17998T=LMG 23454T) is proposed as the type strain of the type species, Rhodonellum psychrophilum sp. nov.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Rhodonellum psychrophilum sp. nov. GCM71T is DQ112660.

Tables detailing carbon source utilization, the results of API 20NE and API ZYM tests and the fatty acid content of the strains analysed in this study and a graph showing the absorbance spectra of strain GCM71T when grown for six weeks with or without light are available as supplementary material in IJSEM Online.

{dagger}Present address: Department of Ecology, Royal Veterinary and Agricultural University, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark.






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Copyright © 2006 by the International Union of Microbiological Societies.