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Int J Syst Evol Microbiol 56 (2006), 2657-2663; DOI  10.1099/ijs.0.63988-0
© 2006 International Union of Microbiological Societies

Pseudomonas moraviensis sp. nov. and Pseudomonas vranovensis sp. nov., soil bacteria isolated on nitroaromatic compounds, and emended description of Pseudomonas asplenii

Ludmila Tvrzová1, Peter Schumann2, Cathrin Spröer2, Ivo Sedlácek3, Zdena Pácová3, Ondrej Sedo4, Zbynek Zdráhal4, Maike Steffen2 and Elke Lang2

1 Department of Microbiology, Institute of Experimental Biology, Faculty of Science, Masaryk University, Tvrdého 14, 602 00 Brno, Czech Republic
2 DSMZ – Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, 38124 Braunschweig, Germany
3 CCM – Czech Collection of Microorganisms, Institute of Experimental Biology, Faculty of Science, Masaryk University, Tvrdého 14, 602 00 Brno, Czech Republic
4 Department of Functional Genomics and Proteomics, Institute of Experimental Biology, Faculty of Science, Masaryk University, Tvrdého 14, 602 00 Brno, Czech Republic

Correspondence
Ludmila Tvrzová
lida{at}sci.muni.cz

Two strains of Gram-negative bacteria isolated from soil by selective enrichment with nitroaromatics were subjected to a polyphasic taxonomic study. On the basis of 16S rRNA gene sequence analysis, the two strains were found to belong to the genus Pseudomonas, within the Gammaproteobacteria. Strain 1B4T shared the highest sequence similarity with Pseudomonas koreensis DSM 16610T (99.5 %) and Pseudomonas jessenii CCM 4840T (99.3 %), and strain 2B2T with Pseudomonas asplenii DSM 17133T (98.9 %), Pseudomonas fuscovaginae DSM 7231T (98.9 %) and Pseudomonas putida DSM 291T (98.7 %). On the basis of phylogenetic analysis, DNA–DNA hybridization and phenotype, including chemotaxonomic characteristics, two novel species, Pseudomonas moraviensis sp. nov. with the type strain 1B4T (=CCM 7280T=DSM 16007T) and Pseudomonas vranovensis sp. nov. with the type strain 2B2T (=CCM 7279T=DSM 16006T), are proposed. The description of P. asplenii was emended on the basis of additional data obtained in this study.

Abbreviations: 3-F-4-NP, 3-fluoro-4-nitrophenol; MALDI-TOF MS, matrix-assisted laser desorption/ionization-time of flight mass spectrometry

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains 2B2T and 1B4T are AY970951 and AY970952, respectively.

A table detailing characteristic peaks obtained by using intact cell MALDI-TOF MS and micrographs showing flagella staining of cells of Pseudomonas moraviensis 1B4T and Pseudomonas vranovensis 2B2T are available as supplementary material in IJSEM Online.






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