HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Extended 16S rRNA gene-based tree and comparison of properties Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Kageyama, A. Articles by Mikami, Y. Search for Related Content PubMed PubMed Citation Articles by Kageyama, A. Articles by Mikami, Y. Agricola Articles by Kageyama, A. Articles by Mikami, Y.

Nocardia shimofusensis sp. nov., isolated from soil, and Nocardia higoensis sp. nov., isolated from a patient with lung nocardiosis in Japan

¹ Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8673, Japan
² Yatsushiro Health Insurance General Hospital, 2-26 Yatsushiro, Kumamoto 866-0862, Japan
³ DSMZ – Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany

Correspondence
Yuzuru Mikami
mikami{at}myco.pf.chiba-u.ac.jp

Three actinomycete strains isolated from soils and one strain isolated from a patient with lung nocardiosis in 1999 and 2001 in Japan have been provisionally assigned to the genus Nocardia on the basis of morphological criteria. These isolates were further investigated to determine their specific taxonomic status. Detailed chemotaxonomic characterization and 16S rRNA gene sequence analysis of these isolates confirmed that they belong to the genus Nocardia. The 16S rRNA gene sequences of the four strains were most similar to that of Nocardia farcinica. However, the sequence similarity values between these four strains and N. farcinica were <98·9 %. These four strains were susceptible to 5-fluorouracil, and they have the ability to decompose urea, which is a very characteristic trait. Furthermore, DNA–DNA relatedness data revealed that IFM 10311^T, IFM 10312 and IFM 10313 comprise a single novel species of Nocardia, that IFM 10084^T represents another novel species of Nocardia and that these two novel species could be distinguished from N. farcinica. The names Nocardia shimofusensis sp. nov. and Nocardia higoensis sp. nov. are proposed, with IFM 10311^T (=NBRC 100134^T=JCM 12122^T=DSM 44733^T) and IFM 10084^T (=NBRC 100133^T=JCM 12121^T=DSM 44732^T) as the respective type strains.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains IFM 10311^T, IFM 10312 and IFM 10313 and IFM 10084^T are AB108775–AB108778, respectively.

An extended 16S rRNA gene-based phylogenetic tree and a comparison of properties of all Nocardia species are available as supplementary material in IJSEM Online.

This article has been cited by other articles:

				P. Kampfer, B. Huber, S. Buczolits, K. Thummes, I. Grun-Wollny, and H.-J. Busse Nocardia acidivorans sp. nov., isolated from soil of the island of Stromboli Int J Syst Evol Microbiol, June 1, 2007; 57(6): 1183 - 1187. [Abstract] [Full Text] [PDF]

				B. A. Brown-Elliott, J. M. Brown, P. S. Conville, and R. J. Wallace Jr Clinical and Laboratory Features of the Nocardia spp. Based on Current Molecular Taxonomy Clin. Microbiol. Rev., April 1, 2006; 19(2): 259 - 282. [Abstract] [Full Text] [PDF]