HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Citing Articles Citing Articles via CrossRef

Microbulbifer variabilis sp. nov. and Microbulbifer epialgicus sp. nov., isolated from Pacific marine algae, possess a rod–coccus cell cycle in association with the growth phase, by M. Nishijima, T. Takadera, N. Imamura, H. Kasai, K.-D. An, K. Adachi, T. Nagao, H. Sano and K. Yamasato

International Journal of Systematic and Evolutionary Microbiology vol. 59, part 7, pp. 1696 - 1707

Supplementary Table S1. Strain isolation details.

Supplementary Table S2. Cellular fatty acid compositions of Microbulbifer variabilis sp. nov. Ni-2088^T, Microbulbifer epialgicus sp. nov. F-104^T and the type strains of Microbulbifer species.

Supplementary Table S3. DNA base composition and DNA–DNA relatedness among the new isolates.

[PDF file of Supplementary Tables S1-S3] (89 KB)

Supplementary Fig. S1. Phylogenetic affiliation of Microbulbifer variabilis sp. nov. Ni-2088^T and Microbulbifer epialgicus sp. nov. F-104^T based on concatenated gyrB and ftsZ gene sequences. [PDF of Supplementary Fig. S1] (26 KB)

Supplementary Fig. S2. Phase-contrast micrographs of two sheets of coccoid cells of M. variabilis sp. nov. Ni-2088^T with different depths of focus. Each sheet of coccoid cells was released from a microcolony on 1/10 MA and floated in 75 % ASW, which was added for microscopic observation. The peripheries of the sheets were at right angles to each other. Bar, 2 µm. Micrographs are focused on the upper sheet (left) and the lower sheet (right).

Supplementary Fig. S3. Phase-contrast micrographs of cells of the type strains of four species of Microbulbifer. [PDF of Supplementary Fig. S3] (6059 KB)

Supplementary Fig. S4. Phase-contrast micrograph of optically dense and faint coccoid cells of M. variabilis sp. nov. Ni-2088^T taken from a culture on 1/10 MA plates maintained at room temperature (23–27 °C) for 14 months.

Supplementary Fig. S5. Transmission electron micrograph of a spherical body of M. variabilis sp. nov. Ni-2088^T formed on the tip of a rod cell. The culture was grown on MA at 30 °C for 4 days and negatively stained with uranyl acetate. Bar, 1 µm.

Supplementary Fig. S6. Dendrogram of fatty acid compositions of M. variabilis sp. nov. Ni-2088^T and 13 other pelagiomicin-producing strains, M. epialgicus sp. nov. F-104^T and the type strains of the seven Microbulbifer species.

Supplementary Fig. S7. Two-dimensional TLC of the polar lipids of M. variabilis sp. nov. Ni-2088^T, M. epialgicus sp. nov. F-104^T and the type strains of four Microbulbifer species.

[PDF file of Supplementary Figs S6 and S7] (66 KB)

This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Citing Articles Citing Articles via CrossRef