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Enterobacter oryzae sp. nov., a nitrogen-fixing bacterium isolated from the wild rice species Oryza latifolia, by G. Peng, W. Zhang, H. Luo, H. Xie, W. Lai and Z. Tan

International Journal of Systematic and Evolutionary Microbiology vol. 59, part 7, pp. 1650 - 1655

Supplementary Fig. S1. Transmission electron micrograph of a cell of strain Ola 51T showing a rod with the flagellar structure. Bar, 0.2 µm.

TEM of a cell of strain Ola 51(T)

Supplementary Fig. S2. IS-PCR fingerprinting patterns of diazotrophic isolates of group A from the wild rice species Oryza latifolia and closely related species. M, Standard DNA marker; lanes 1–9, Enterobacter aerogenes ATCC 13048T, Enterobacter cloacae ATCC 13047T, Enterobacter radicincitans DSM 16656T and group A isolates Ola 51T, Ola 10, Ola 50, Ola 12, Ola 01 and Ola 28.

IS-PCR fingerprinting of novel strains and related reference strains

Supplementary Fig. S3. Whole-cell protein SDS-PAGE patterns of diazotrophic isolates of group A from O. latifolia and closely related reference strains. M, Molecular-mass standards; lanes 1–8, E. aerogenes ATCC 13048T, E. cloacae ATCC 13047T and group A isolates Ola 51T, Ola 10, Ola 50, Ola 12, Ola 01 and Ola 28. Arrows indicate intense protein bands that differ between E. cloacae ATCC 13047T and the diazotrophic isolates.

Whole-cell protein SDS-PAGE of novel strains and related reference strains

Supplementary Fig. S4. Neighbour-joining dendrogram based on rpoB gene sequences of Enterobacter oryzae sp. nov. Ola 51T and closely related Enterobacter species. [PDF] (30 KB)

Supplementary Table S1. Biological and biochemical characteristics of strain Ola 51T, E. radicincitans DSM 16656T and E. cloacae ATCC 13047T.

Supplementary Table S2. Cellular fatty acid composition determined as methyl esters by gas chromatography.

Supplementary Table S3. DNA–DNA relatedness (%) among novel diazotrophic strains isolated from wild rice and some reference strains.

[PDF file of Supplementary Tables S1-S3] (98 KB)







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