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Bifidobacterium mongoliense sp. nov., from airag, a traditional fermented mare’s milk product from Mongolia, by K. Watanabe, H. Makino, M. Sasamoto, Y. Kudo, J. Fujimoto and S. Demberel

International Journal of Systematic and Evolutionary Microbiology vol. 59, part 6, pp. 1535 - 1540

Supplementary Fig. S1. RAPD-PCR fingerprinting of strains YIT 10443T (lanes 1) and YIT 10738 (lanes 2). Three primers with random sequences (A, 5′-CCGCAGCCA A-3′; B, 5′-AACGCGCAAC-3′; C, 5′-GCGGAAATAG-3′) were used. Lane M, 100 bp ladder DNA size marker (Seegene).

RAPD-PCR profiles of strains YIT 10443(T) and YIT 10738

Supplementary Fig. S2. Phylogenetic tree based on 16S rRNA gene sequences constructed by using the maximum-likelihood method based on comparison of approximately 1460 positions. [PDF] (63 KB)

Supplementary Fig. S3. Scanning electron micrographs of cells of Bifidobacterium mongoliense sp. nov. YIT 10443T (a, b) and B. mongoliense sp. nov. YIT 10738 (c, d). (a, c) Cells grown in modified GAM broth overnight at 30 °C under anaerobic conditions; (b, d) cells grown on modified GAM agar plate at 30 °C for 3 days under aerobic conditions. Bars, 1 µm.

SEMs of cells of strains YIT 10443(T) and YIT 10738







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