Glaciecola lipolytica sp. nov., isolated from seawater near Tianjin city, China, by L.-P. Chen, H.-Y. Xu, S.-Z. Fu, H.-X. Fan, Y.-H. Liu, S.-J. Liu and Z.-P. Liu
International Journal of Systematic and Evolutionary Microbiology vol. 59, part 1, pp. 73 - 76
Supplementary Fig. S1. Transmission electron micrographs of cells of strain E3T after negative staining with 1 % (w/v) phosphotungstic acid, showing flagella (F), prosthecae (P) and buds (B). Cells were grown on MA at 30 °C for 2 days. Bars, 500 nm.
Supplementary Fig. S2. Polar lipid profile of strain E3T. Polar lipids were examined by two-dimensional TLC and characterized with spraying reagents specific for α-glycols (periodate–Schiff), sugars (α-naphthol/sulfuric acid, anisaldehyde/sulfuric acid), free amino groups (ninhydrin) and phosphate (Zindzadze). A 50 % sulfuric acid solution was used to detect spots of all kinds of lipids. bis-P, bis-phosphatidic acid; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; GL1, unknown glycolipid.
Supplementary Table S1. Comparison of cellular fatty acid profile of strain E3T with those of type strains of Glaciecola species. [PDF] (70 KB)