Description of seven candidate species affiliated with the phylum Actinobacteria, representing planktonic freshwater bacteria, by M. W. Hahn
International Journal of Systematic and Evolutionary Microbiology vol. 59, part 1, pp. 112 - 117
Supplementary Fig. S1. Growth of 'Candidatus Limnoluna rubra' strain MWH-EgelM2-3 and an accompanying non-actinobacterial strain on NSY agar plates. The colonies of the candidate species are red-pigmented and those of the other strain are unpigmented. The plated liquid culture contained about 109 cells ml–1 and was dominated by the actinobacterial strain (>90 % of cells). The photo shows a section of a plate that received an inoculum of about 105 cells. The strain of the candidate species grows well when in direct contact with the non-pigmented colonies; however, the size of the actinobacterial colonies decreases with distance from the non-pigmented colonies and, beyond a crucial distance from these colonies, no actinobacterial growth occurs.
Supplementary Fig. S2. Neighbour-joining tree based on almost full-length 16S rRNA gene sequences (E. coli positions 47–1465). The tree reconstructs the phylogenetic relationships between the novel candidate species and environmental sequences of uncultured actinobacteria. [PDF] (510 KB)
Supplementary Table S1. Pairwise comparison of the sequence similarity of 16S rRNA genes of the candidate species.
Supplementary Table S2. Geographical, limnological and climatic characteristics of habitats from which the strains representing the seven candidate species were isolated.
[PDF file of Supplementary Tables S1 and S2] (68 KB)
