Caenimonas koreensis gen. nov., sp. nov., isolated from activated sludge, by S. H. Ryu, D. S. Lee, M. Park, Q. Wang, H. H. Jang, W. Park and C. O. Jeon
International Journal of Systematic and Evolutionary Microbiology vol. 58, part 5, pp. 1064 - 1068
Supplementary Fig. S1. Transmission electron micrograph showing the general morphology of negatively stained cells of strain EMB320T after growth for 5 days at 30 °C on R2A broth. Bar, 1 µm.
Supplementary Fig. S2. Polar lipid TLC sprayed with molybdatophosphoric acid reagent of strain EMB320T. Ascending solvent system: (I) chloroform/methanol/water (65:25:4, by vol.); (II) chloroform/acetic acid/methanol/water (80:13:10:2, by vol.). For detection of polar lipids, molybdatophosphoric acid (PE, PG, DPG and AL), ninhydrin (PE and AL) and molybdenum blue reagent (PG, PE and DPG) were applied. The unknown was inferred as an amino group-containing lipid. PE, Phosphatidylethanolamine; PG, phosphatidylglycerol; DPG, diphosphatidylglycerol; AL, amino group-containing lipid.
Supplementary Fig. S3. Maximum-likelihood (a) and maximum-parsimony (b) trees showing phylogenetic relationships of strain EMB320T and related taxa. Burkholderia cepacia ATCC 25416T was used as an outgroup. Bars, 0.1 changes per nucleotide position. [PDF] (21 KB)
