Halobacillus alkaliphilus sp. nov., a halophilic bacterium isolated from a salt lake in Fuente de Piedra, southern Spain, by I. Romano, I. Finore, G. Nicolaus, F. J. Huertas, L. Lama, B. Nicolaus and A. Poli
International Journal of Systematic and Evolutionary Microbiology vol. 58, part 4, pp. 886 - 890
Supplementary Fig. S1. Scanning electronic micrograph of cells of strain FP5T. Bar, 5 µm.
Supplementary Fig. S2. Total polar lipids of strain FP5T. (A) Two-dimensional TLC of phospholipids detected by spraying the plate with Dittmer and Lester reagent. (B) Two-dimensional TLC of glycolipids detected by spraying the plate with α-naphthol reagent. The solvent system was chloroform/methanol/water (65:25:4 by vol.) for the first dimension and chloroform/methanol/acetic acid/water (85:15:12:4 by vol.) for the second dimension. (C) TLC of total polar lipids; the right half was sprayed with α-naphthol reagent and the left half was sprayed with Dittmer and Lester reagent. The solvent system was chloroform/methanol/water (65:25:4 by vol.).
