Natrinema gari sp. nov., a halophilic archaeon isolated from fish sauce in Thailand, by W. Tapingkae, S. Tanasupawat, T. Itoh,, K. L. Parkin,, S. Benjakul, W. Visessanguan and R. Valyasevi
International Journal of Systematic and Evolutionary Microbiology vol. 58, part 10, pp. 2378 - 2383
Supplementary Fig. S1. Two-dimensional TLC of polar lipids extracted from strain HIS40-3T (a) and strain HDS3-1 (b). Separation of components was achieved by developing the plate with chloroform/methanol/water (65:25:4 by vol., horizontal direction) and then with chloroform/methanol/acetic acid/water (85:12:15:4 by vol., vertical). Spots were visualized by staining with anisaldehyde reagent and the plate was heated to 150 °C for 10 min to show glycolipids and phospholipids. PG, Phosphatidylglycerol; PGP-Me, phosphatidylglycerol phosphate methyl ester; PGS, phosphatidylglycerol sulfate; GL, unidentified glycolipid; Un, unknown lipid; Pi, pigment.
Supplementary Fig. S2. Phylogenetic trees showing the relationships between strains HIS40-3T and HDS3-1 and related archaeal species based on 16S rRNA gene sequences. [PDF] (20 KB)
Supplementary Fig. S3. Whole-cell proteins extracted from strains HIS40-3T and HDS3-1 and strains of related Natrinema species, following separation by SDS-PAGE (10 %). Proteins were stained with Coomassie blue. Lanes: 1, molecular mass markers; 2, Nnm. pallidum JCM 8980T; 3, Nnm. pellirubrum JCM 10476T; 4, Nnm. altunense JCM 12890T; 5, strain HIS40-3T; 6, strain HDS3-1. Arrows indicated major differences in protein bands.
