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Supplementary data

Nocardioides hwasunensis sp. nov., by Soon Dong Lee, Dong Wan Lee and Jung-Sup Kim

International Journal of Systematic and Evolutionary Microbiology vol. 58, part 1, pp. 278-281

Table S1. Cellular fatty acid composition (%) of strains HFW-18 and HFW-21T.

Fig. S1. A transmission electron micrograph of a cell of strain HFW-21T grown on trypticase soy broth for 24 h at 30 °C. Bar, 0.2 μm.

Fig. S2. Neighbour-joining tree (Saitou & Nei, 1987) showing phylogenetic positions of strains HFW-18 and HFW-21T within the radiation encompassing representatives of the family Nocardioidaceae. Tree construction was based on a total of 1348 nt in unambiguously aligned positions of 16S rRNA gene sequences. Streptomyces griseus KCTC 9080T (M76388) was used as an outgroup. Asterisks at the corresponding branches are those also found in both maximum-likelihood (Felsenstein, 1981) and maximum-parsimony (Fitch, 1971) trees. Numbers at the nodes are percentages of bootstrap support (>50 %), based on a neighbour-joining analysis of 1000 resamplings. Bar, 1 substitution per 100 nt positions.

References

Felsenstein, J. (1981). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368-376.

Fitch, W. M. (1971). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406-416.

Saitou, N. & Nei, M. (1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406-425.







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