Methylocystis hirsuta sp. nov., a novel methanotroph isolated from a groundwater aquifer, by A. S. Lindner, A. Pacheco, H. C. Aldrich, A. Costello Staniec, I. Uz and D. J. Hodson
International Journal of Systematic and Evolutionary Microbiology vol. 57, part 8, pp. 1891 - 1900
Supplementary Fig. S1. Phylogenetic relationships of the partial mmoX and mxaF gene sequences of strain CSC1T and members of related genera. [PDF] (19 KB)
Supplementary Fig. S2. Electron microscope cytochemistry of the S-layer of strain CSC1T. (a) Surface of a cell fixed initially with glutaraldehyde alone. The spiny layer is indistinct and lightly stained. (b) Surface of a cell fixed initially with a glutaraldehyde/Alcian blue mixture to stain polysaccharide selectively. Compared with (a), the spiny layer stains darker and is thicker and is more distinct. (c, d) Cells after Pronase digestion. In (c), a cross-section, a light layer around the cell has been left where the Pronase has removed the protein in the S-layer. In (d), a grazing section of the spines at the cell surface, numerous light spots in the plastic show where the Pronase has removed the spikes. Bars, 0.2 µm.
