'Candidatus Magnetoglobus multicellularis', a multicellular, magnetotactic prokaryote from a hypersaline environment, by F. Abreu, J. L. Martins, T. S. Silveira, C. N. Keim, H. G. P. Lins de Barros, F. J. Gueiros Filho and U. Lins
International Journal of Systematic and Evolutionary Microbiology vol. 57, part 6, pp. 1318 - 1322
Supplementary Fig. S1. (A) Distribution of 'Candidatus Magnetoglobus multicellularis' based on forward (size) and side (cell complexity) scatter detection in the flow cytometer. Note the presence of two populations. The scanning electron microscopy images represent these micro-organisms in different stages of their life cycle. (B) Cell size (forward scatter) and associated fluorescence of 10000 'Candidatus Magnetoglobus multicellularis' aggregates per sample measured in fluorescence channel-1 (FL1-H), detecting glutaraldehyde fluorescence associated with protein content.
Supplementary Fig. S2. FISH with 'Candidatus Magnetoglobus multicellularis'-specific probe (rhodamine; right) and DAPI stain of the same cells (left). Bar, 10 µm.
Supplementary Fig. S3. Fluorescence microscopy of 'Candidatus Magnetoglobus multicellularis' stained with the LIVE/DEAD BacLightTM kit. Note that live cells stain green while, during disaggregation, individual cells are dead (red). Bar, 10 µm.
