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Rhizobium cellulosilyticum sp. nov., isolated from sawdust of Populus alba, by P. García-Fraile, R. Rivas, A. Willems, A. Peix, M. Martens, E. Martínez-Molina, P. F. Mateos and E. Velázquez

International Journal of Systematic and Evolutionary Microbiology vol. 57, part 4, pp. 844 - 848

Supplementary Fig. S1. Cellulase activity presented by strain ALA10B2T on CEA plates after 5 days incubation at 28 °C.

Cellulase activity of strain ALA10B2(T)

Supplementary Fig. S2. TP-RAPD profiles obtained by using primers 879F and 1522R. Lanes: 1, Rhizobium cellulosilyticum sp. nov. ALA10B2T; 2, R. cellulosilyticum sp. nov. ALA38.2; 3, R. galegae USDA 4128T; 4, R. huautlense SO2T. Lane MW, standard VI of Boehringer-Roche (sizes in bp).

TP-RAPD profiles of strain ALA10B2(T) and related strains

Supplementary Fig. S3. Neighbour-joining tree based on nearly complete 16S rRNA gene sequences of strain ALA10B2T and other related organisms of the family Rhizobiaceae, including an extended selection of reference organisms.

Supplementary Fig. S4. Unrooted neighbour-joining tree based on partial atpD sequences of strain ALA10B2T and close relatives.

Supplementary Fig. S5. Unrooted neighbour-joining tree based on partial recA sequences of the novel strains and close relatives.

[PDF file of Supplementary Figs S3-S5] (25 KB)

Supplementary Fig. S6. Nodules induced by strain ALA10B2T (a) and Ensifer meliloti GR4 (b) on Medicago sativa roots. Bars, 0.3 cm.

Nodulation by strain ALA10B2(T) and E. meliloti GR4

Supplementary Fig. S7. Plasmid profiles in a horizontal 0.7 % agarose gel. Lanes: 1, Ensifer meliloti GR4; 2, strain ALA10B2T. Results of hybridization using a nodD probe: lane 3, E. meliloti GR4; 4, strain ALA10B2T (marked by an arrow).

Plasmid profiles of strain ALA10B2(T) and E. meliloti GR4

Supplementary Fig. S8. Comparative sequence analysis of nodD gene sequences from strain ALA10B2T and representative related strains from GenBank. [PDF] (15 KB)

Supplementary Table S1. Results of DNA–DNA hybridization experiments

Strain providing fixed DNAStrain used for labelled probe
1234
1. R. galegae USDA 4128T100272018
2. R. huautlense SO2T221001421
3. R. cellulosilyticum ALA10B2T1025100100
4. R. cellulosilyticum ALA38.21514100100




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