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Ochrobactrum cytisi sp. nov., isolated from nodules of Cytisus scoparius in Spain, by J. L. Zurdo-Piñeiro, R. Rivas, M. E. Trujillo, N. Vizcaíno, J. A. Carrasco, M. Chamber, A. Palomares, P. F. Mateos, E. Martínez-Molina and E. Velázquez

International Journal of Systematic and Evolutionary Microbiology vol. 57, part 4, pp. 784 - 788

Supplementary Fig. S1. TP-RAPD profiles obtained by using the primer pairs 27F and 1522R (lanes 1–5) and 879F and 1522R (lanes 6–10).Lanes: 1 and 6, strain ESC1T; 2 and 7, strain ESC5; 3 and 8, O. tritici LMG 18957T; 4 and 9, O. anthropi LMG 3331T; 5 and 10, O. lupini LUP21T. Lanes MW, standard VI of Boehringer-Roche.

TP-RAPD profiles

Supplementary Figs S2 and S3. Comparative sequence analysis of nifH gene sequences (320 nt) (Fig. S2) and nodD gene sequences (296 nt) of strain ESC1T and representative strains that nodulate legumes. [PDF] (17 KB)

Supplementary Fig. S4. Nodules induced by strain ESC1T (a) and Rhizobium etli CFN42T (b) on Phaseolus vulgaris roots. Bars, 0.3 cm.

Nodules on Phaseolus vulgaris roots

Supplementary Table S1. Fatty acid profiles of the novel strains compared with those of the closest phylogenetically related species of genus Ochrobactrum.

Supplementary Table S2. Differentiating physiological characters between the novel strains (O. cytisi sp. nov.) and the remaining species of genus Ochrobactrum.

[PDF file of Supplementary Tables S1 and S2] (35 KB)







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