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Emended description of the genus Phyllobacterium and description of four novel species associated with plant roots: Phyllobacterium bourgognense sp. nov., Phyllobacterium ifriqiyense sp. nov., Phyllobacterium leguminum sp. nov. and Phyllobacterium brassicacearum sp. nov., by S. Mantelin, M. Fischer-Le Saux, F. Zakhia, G. Béna, S. Bonneau, H. Jeder, P. de Lajudie and J.-C. Cleyet-Marel

International Journal of Systematic and Evolutionary Microbiology vol. 56, part 4, pp. 827 - 839

Composition of media used

YM medium (l^-1):
1 g yeast extract; 0.5 g sodium glutamate; 10 g D-mannitol; 0.5 g KH₂PO₄; 0.1 g MgSO₄.7H₂O; 1 ml 0.4 % (w/v) FeCl₃; 1 ml 5 % (w/v) NaCl; 1 ml 4 % (w/v) CaCl₂; pH 6.8.

Recommended medium for growth and maintenance of Ochrobactrum anthropi (l^-1):
1 g beef extract; 2 g yeast extract; 5 g peptone; 5 g NaCl; pH 7.4.

Recommended medium for growth and maintenance of Chelatobacter heintzii (l^-1):
1 g beef extract; 2 g yeast extract; 5 g peptone; 0.45 g KH₂PO₄; 2.39 g Na₂HPO₄.12H₂O; 5 g NaCl; pH 6.8.

Recommended medium for growth and maintenance of Rhodobacter sphaeroides (l^-1):
1 g yeast extract; 1 g disodium succinate; 5 ml 0.1 % (w/v) ferric citrate solution; 0.5 g KH₂PO₄; 0.4 g MgSO₄.7H₂O; 0.4 g NaCl; 0.4 g NH₄Cl; 50 mg CaCl₂.2H₂O; 1 ml trace element solution (see below); 0.5 ml ethanol; pH 6.8.

Trace element solution (l^-1):
0.1 g ZnSO₄.7H₂O; 30 mg MnCl₂.4H₂O; 0.3 g H₃BO₃; 0.2 g CoCl₂.6H₂O; 10 mg CuCl₂.2H₂O; 20 mg NiCl₂.6H₂O; 30 mg Na₂MoO₄.2H₂O.

Distribute 40 ml medium into 50 ml screw-capped bottles. Flush each bottle for 1 to 2 min with nitrogen gas and then close immediately with rubber septa and screw-caps. Autoclave (120 °C, 20 min). Sterile syringes are used to inoculate and remove the samples. Incubate in light using a tungsten lamp.

Sequence alignments

Alignment 1. Sequence alignment for the ssu (16S rRNA) gene phylogeny. ijs63911_SuppData1.pir (94 KB)

Alignment 2. Sequence alignment for the atpD gene phylogeny. ijs63911_SuppData2.pir (26 KB)

Extended phylogenetic trees

Supplementary Fig. S1. Complete distance tree based on 16S rRNA gene sequences. [PDF] (260 KB)

Supplementary Fig. S2. Complete distance tree based on atpD gene sequences. [PDF] (259 KB)

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