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Rhizobium lusitanum sp. nov., a bacterium that nodulates Phaseolus vulgaris, by A. Valverde, J. M. Igual, A. Peix, E. Cervantes and E. Velázquez

International Journal of Systematic and Evolutionary Microbiology vol. 56, part 11, pp. 2631 - 2637

Supplementary Fig. S1. Plasmid profiles of the isolates from this study.

Supplementary Figs S2 and S3. Comparative analysis of nodD (Fig. S2) and nifH (Fig. S3) gene sequences from Rhizobium lusitanum sp. nov. P1-7T and representative related strains from GenBank.

Supplementary Figs S4 and S5. RAPD (Fig. S4) and TP-RAPD (Fig. S5) patterns of isolates from this study.

Supplementary Fig. S6. Neighbour-joining tree based on nearly complete 16S rRNA gene sequences of strain P1-7T (R. lusitanum sp. nov.) and other related organisms of the family Rhizobiaceae.

Supplementary Figs S7 and S8. Neighbour-joining trees based on partial recA (Fig. S7) and atpD (Fig. S8) gene sequences of strains of R. lusitanum sp. nov. and other related organisms.

Supplementary Fig. S9. ITS fragments of various strains.

Supplementary Fig. S10. Neighbour-joining tree based on 16S–23S rRNA intergenic sequences of R. lusitanum sp. nov. strains and phylogenetically related species of the genus Rhizobium.

Supplementary Fig. S11. Low-molecular-weight RNA profiles of R. lusitanum sp. nov. P1-7T and related strains.

Supplementary Table S1. Results of DNA-DNA hybridization experiments.

[PDF file of Supplementary Figs S1-S11 and Supplementary Table S1] (1145 KB)







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