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Byssovorax cruenta gen. nov., sp. nov., nom. rev., a cellulose-degrading myxobacterium: rediscovery of ‘Myxococcus cruentus’ Thaxter 1897, by H. Reichenbach, E. Lang, P. Schumann and C. Spröer

International Journal of Systematic and Evolutionary Microbiology vol. 56, part 10, pp. 2357 - 2363

Migrating pseudoplasmodia

Supplementary Fig. S1. Migrating pseudoplasmodia of Byssovorax cruenta By c2T deposit wrinkled, parchment-like slime tracks. Bar, 570 µm.

   Knob of piled up vegetative cells

Supplementary Fig. S2. A knob of piled up vegetative cells of the myxobacterium Byssovorax cruenta By c2T, resembling a Myxococcus fruiting body. On the surface of a water agar plate with a streak of autoclaved Escherichia coli. The diameter of the base of the mass is approximately 140 µm.


Pseudoplasmodium becoming a ring structure   Rare cup-shaped structure

Supplementary Fig. S3. Instead of knobs, the pseudoplasmodia of Byssovorax cruenta By c2T often end up in large, red rings, this one near the edge of a piece of filter paper. Bar, 100 µm.


  

Supplementary Fig. S4. Rarely, cup-shaped structures arise from Byssovorax cruenta By c2T. Bar, 380 µm.

Polyangium strain resembling Byssovorax

Supplementary Fig. S5. Sometimes, strains are isolated (in this case strain Pl 10973), tentatively identified as Polyangium, that also grow as pseudoplasmodia resembling those of Byssovorax. Bar, 260 µm.

   Coherent swarms

Supplementary Fig. S6. Growth of Byssovorax cruenta By c2T in the form of a coherent swarm emerging from an agar block used as the inoculum (top) and from the edge of a piece of macerated filter paper on yeast agar (bottom). Typical myxobacterial swarm colonies with long, radial veins have developed. Bars, 2150 µm.

Single sporangiole   Crystals formed by strain By c2(T)

Supplementary Fig. S7. Single sporangiole of Byssovorax cruenta By c2T within an additional outer wall. Bar, 25 µm.

  

Supplementary Fig. S8. Crystals in macerated filter paper arising from cultures of Byssovorax cruenta By c2T. Bar, 30 µm.


Red oil droplets   Colony of strain By c2(T)

Supplementary Fig. S9. Intensely red oil droplets in a degenerated culture of Byssovorax cruenta By c2T. Bar, 13 µm.

  

Supplementary Fig. S10. Colony of Byssovorax cruenta By c2T on filter paper placed on mineral salts agar showing decomposition of cellulose. Bar, 1100 µm.







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