Novosphingobium lentum sp. nov., a psychrotolerant bacterium from a polychlorophenol bioremediation process, by M. A. Tiirola, H.-J. Busse, P. Kämpfer and M. Männistö
International Journal of Systematic and Evolutionary Microbiology vol. 55, part 2, pp. 583 - 588
Supplementary Figs A–C. Phylogenetic trees calculated using the neighbour-joining method (A), maximum-parsimony method (B) and maximum-likelihood method (C) indicating phylogenetic relationships between strain MT1T (SSP303009) and other members of the genus Novosphingobium. The trees are based on a 1375 bp alignment of 16S rRNA gene sequences. The type strains of Sphingomonas paucimobilis, Sphingomonas suberifaciens and Sphingobium yanoikuyae were chosen as outgroups. Bootstrap percentages based on 1000 replications are indicated. Scale bars in (A) and (C) indicate 1 substitution per 100 nucleotides. [PDF of Figs A-C] (23 KB)
Supplementary Fig. D. Euclidean distance dendrogram based on the whole-cell fatty acid compositions of strains MT1T, MT101, MT103 and MT104 and eight type strains of the genus Novosphingobium (N. tardaugens JCM 11434T, N. hassiacum DSM 14552T, N. rosa IFO 15208T, N. capsulatum ATCC 14666T, N. stygium SMCC B0712T, N. subterraneum SMCC B0478T, N. aromaticivorans SMCC F199T and N. subarcticum HAMBI 2110T).
Supplementary Fig. E. Polar lipid profile of MT1T after two-dimensional TLC and detection with molybdatophosphoric acid. Abbreviations: PE, phosphatidylethanolamine; PG, phosphatidylglycerol; DPG, diphosphatidylglycerol; PC, phosphatidylcholine; SGL, sphingoglycolipid; PME, phosphatidylmonomethylethanolamine; PDE, phosphatidyldimethylethanolamine; PLx, unknown phospholipid; PGLx, unknown phosphoglycolipid; GLx, unknown glycolipid; Lx, unknown polar lipid. [PDF of Figs D and E] (114 KB)
Supplementary Table A. Whole-cell fatty acid composition of the members of the genus Novosphingobium and strains MT1T, MT101, MT103 and MT104 grown in R2A broth at 22 °C. [PDF] (18 KB)
