| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
-proteobacterium isolated from hypersaline Ekho Lake, Antarctica
1 Institut für Allgemeine Mikrobiologie, Christian-Albrechts-Universität, Kiel, Germany
2 School of Food Biosciences, University of Reading, PO Box 226, Reading RG6 6AP, UK
3 DSMZ – Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, D-38124 Braunschweig, Germany
Correspondence
Matthias Labrenz
matthias.labrenz{at}io-warnemuende.de
 |
ABSTRACT |
|---|
 TOP ABSTRACT MAIN TEXTREFERENCES |
|---|
7c, with 3-OH 10 : 0, 16 : 17c and 18 : 0 in lower amounts. The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylcholine. Ubiquinone 10 was produced. The DNA G+C content was 67 mol%. 16S rRNA gene sequence comparisons indicated that the isolate represents a member of the Roseobacter clade within the 
-Proteobacteria. The organism showed no particular relationship to any members of this clade but clustered on the periphery of the genera Jannaschia, Octadecabacter and ‘Marinosulfonomonas’ and the species Ruegeria gelatinovorans. Distinct morphological, physiological and genotypic differences to these previously described taxa supported the description of a new genus and a novel species, for which the name Roseisalinus antarcticus gen. nov., sp. nov. is proposed. The type strain is EL-88T (=DSM 11466T=CECT 7023T).
Published online ahead of print on 30 July 2004 as DOI 10.1099/ijs.0.63230-0.
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain EL-88T is AJ605747.
A figure showing characteristic absorbance peaks for strain EL-88T is available as supplementary material in IJSEM Online.
Present address: IOW – Baltic Sea Research Institute Warnemuende, Seestrasse 15, 18119 Rostock-Warnemuende, Germany. 
|
MAIN TEXT |
|---|
|
TOPABSTRACTMAIN TEXTREFERENCES |
|---|
). Moreover, phototrophy based on bchl a-mediated ‘aerobic anoxygenic photosynthesis' has been estimated to account for up to 5 % of surface ocean photosynthetic electron transport and 11 % of the total microbial community (Béjà et al., 2002). Ekho Lake, East Antarctica, is a hypersaline, meromictic and heliothermal lake that contains, in its deeper layers, a microflora probably of marine origin (Labrenz & Hirsch, 2001). In an attempt to elucidate the microbial diversity of Ekho Lake, two aerobic bchl a-producing genera, Roseovarius and Staleya, have been described previously by our laboratories (Labrenz et al., 1999, 2000). Here, we present data on a novel aerobic bchl a-producing taxon isolated from the lake.
One bacterial isolate was obtained from a 10 m Ekho Lake sample; at this depth, salinity was 70 
, temperature was 15·5 °C and pH was 8·01. This isolate is referred to as EL-88T. Enrichment and isolation of this strain were performed as described by Labrenz et al. (1998), and enrichment conditions followed characteristics of the original water samples. Pure cultures were kept as serial transfers on slants, lyophilized or deep-frozen at –72 °C in glycerol. Morphological, physiological and metabolic analyses were performed as described in detail by Labrenz et al. (1998, 1999, 2000, 2003) and Lawson et al. (2000).
Analysis of fatty acid methyl esters was carried out with 20 mg freeze-dried biomass and using methods that allowed selective hydrolysis of ester- and amide-linked fatty acids as described previously (Labrenz et al., 2000). Respiratory lipoquinones and polar lipids were extracted from 100 mg freeze-dried material using the two-stage method and analysed according to the methods of Tindall (1990a, b). Cell-wall diamino acids were separated by one-dimensional TLC on cellulose plates using the solvent system of Rhuland et al. (1955). DNA G+C contents were analysed according to Mesbah et al. (1989) as described by us previously (Labrenz et al., 1998).
16S rRNA gene sequence fragments were generated by PCR using universal primers pA (positions 8–28 of the Escherichia coli numbering) and pH* (1542–1522). The amplified products were purified using a QIAquick PCR Purification Kit (Qiagen) and sequenced directly using primers to conserved regions of the rRNA gene. Sequencing was performed using a PRISM Taq Dyedeoxy Terminator Cycle Sequencing Kit and an automatic DNA sequencer (model 373A, both from Applied Biosystems). To establish the closest relatives to the strain EL-88T, preliminary searches in the EMBL database were performed with the program FASTA (Pearson & Lipman, 1988). Closely related sequences were retrieved from EMBL and aligned with the newly determined sequences using the program DNATOOLS (Rasmussen, 1995). The resulting multiple sequence alignment had approximately 100 bases at the 5' end of the molecule omitted from further analysis because of alignment uncertainties resulting from the highly variable region V1, using the program GENEDOC (Nicholas et al., 1997). A phylogenetic tree was constructed according to the neighbour-joining method (Saitou & Nei, 1987) with the programs DNATOOLS and TreeView (Page, 1996), and the stability of the groupings was estimated by bootstrap analysis (1000 replications).
The isolate was a motile Gram-negative rod with one or both cell poles narrower (Fig. 1a–c). However, neither position nor number of flagella could be determined. Holdfast structures were often produced (Fig. 1d) and cells had a strong tendency to form rosettes (Fig. 1). Spores were not produced. Polymers were probably secreted (Fig. 1d), but this was not analysed further. Poly-
-hydroxybutyrate was present. Cell growth appeared to be monopolar because one cell end was usually narrower and shorter, possibly indicating a budding process. Cell size was in the range 0·90–1·02x2·18–4·20 µm with a mean size of 0·96x3·19 µm.
|
, and references herein) plus 25 artificial sea water (ASW) (Lyman & Fleming, 1940). Colonies were 2 mm in diameter, circular with regular edges, smooth, convex and red. Growth in liquid cultures occurred as small aggregates. The temperature range for growth was < 3–33·5 °C. Optimal growth occurred between 16 and 26 °C at pH 5·5–9·0. Optimal pH for growth was 7·0–7·8. Requirements for Na+, Cl–, K+, Mg2+, Ca2+ or 
were studied in PYGV+ASW, where Na+ was exchanged with K+, Mg2+ with Ca2+, Cl– with 
and vice versa. The isolate had an absolute requirement for Na+ as well as for Cl–; K+, Mg2+, Ca2+ and 
could be replaced by other ions. Tolerance for NaCl could not be detected because growth was not detected without ASW. Osmotolerance ranged from 10 to 130 ASW, with optimum growth at 50–90 . Growth did not occur on glucose anaerobically in the absence of nitrate. Cells did not grow photoautotrophically with H2/CO2 (80 : 20) or photo-organotrophically with acetate or glutamate. Strain EL-88T exhibited peroxidase, catalase and cytochrome oxidase activity. Cell growth did not depend on vitamins. Weak assimilatory nitrate reduction to nitrite occurred.
Growth of the isolate on different carbon sources as well as further characteristics are given in the species description. The isolate did not grow in a minimal medium (Labrenz et al., 1998) with 0·2 % (w/v) methanol or citric acid. With the API 50CH system the following carbon sources were not metabolized: erythritol, adonitol, methyl -D-xyloside, D-mannose, L-sorbose, dulcitol, sorbitol, methyl -D-mannoside, methyl -D-glucoside, N-acetylglucosamine, amygdalin, arbutin, salicin, lactose, trehalose, inulin, melezitose, D-raffinose, amidone, glycogen, xylitol, D-turanose, D-tagatose, L-arabitol, gluconic acid and 2- as well as 5-ketogluconic acid. In the Biolog system, the isolate did not metabolize -cyclodextrin, dextrin, glycogen, Tween 80, N-acetyl-D-galactosamine, N-acetylglucosamine, adonitol, L-arabinose, D-arabitol, cellobiose, i-erythritol, D-fructose, L-fucose, D-galactose, gentiobiose, -D-glucose, m-inositol, -lactose, -D-lactose-lactulose, maltose, D-mannitol, D-mannose, D-melibiose, methyl -D-glucoside, psicose, D-raffinose, L-rhamnose, D-sorbitol, sucrose, D-trehalose, turanose, xylitol, methylpyruvate, monomethylsuccinate, cis-aconitic acid, formic acid, D-galactonic acid, lactone, D-galacturonic acid, D-gluconic acid, D-glucosaminic acid, D-glucuronic acid, -hydroxybutyric acid, -hydroxybutyric acid, 
-hydroxybutyric acid, p-hydroxyphenylacetic acid, -ketobutyric acid, -ketoglutaric acid, -ketovaleric acid, DL-lactic acid, malonic acid, quinic acid, sebacic acid, succinic acid, succinamic acid, glucuronamide, alaninamide, D-alanine, L-alanine, L-alanylglycine, L-asparagine, L-aspartic acid, L-glutamic acid, glycyl L-aspartic acid, glycyl-L-glutamic acid, L-histidine, hydroxy-L-proline, L-leucine, L-ornithine, L-phenylalanine, L-proline, L-pyroglutamic acid, D-serine, L-serine, L-threonine, DL-carnitine, -aminobutyric acid, urocanic acid, inosine, uridine, phenylethylamine, putrescine, 2-aminoethanol, 2,3-butanediol, glycerol, DL--glycerophosphate, glucose 1-phosphate or glucose 6-phosphate.
Bchl a was present in cell suspensions of strain EL-88T grown aerobically in sporadic dim light. Characteristic absorbance values were found, with a larger peak at 870 nm and smaller peaks at 800–801 nm and 590–592 nm (data available as supplementary material in IJSEM Online). These differed from the maxima of bchl a-containing Roseobacter denitrificans, Roseobacter litoralis, Staleya guttiformis and Roseovarius tolerans (Table 1). Other features, such as carotenoids, were not characterized further. Unlike for Roseobacter denitrificans, vesicular structures of intracytoplasmic membrane systems (Harashima et al., 1982) were not found in ultra-thin sections of aerobically grown cells (Fig. 1d
).
|
7c, with 3-OH 10 : 0, 16 : 17c and 18 : 0 present in smaller amounts. Fatty acids 16 : 17c and 18 : 0 were released by methods which indicated that they were amide-linked. The presence of ubiquinone 10 as the dominant respiratory lipoquinone is characteristic of members of the -Proteobacteria. The predominant fatty acid 18 : 17c, accounting for approximately 62 % of the total fatty acids, is a feature characteristic of several major phyletic groups within the -Proteobacteria. The presence of 3-OH 10 : 0 is indicative that the novel isolate belonged within the same phyletic group as members of the genera Jannaschia, Octadecabacter, Staleya, Sulfitobacter and Roseobacter.
The DNA G+C content of the newly isolated strain was found to be 66·8–66·9 mol%. Characteristics differentiating strain EL-88T from other related organisms are shown in Table 2.
|
-Proteobacteria (data not shown). Pairwise analysis revealed that the novel isolate displayed highest 16S rRNA gene sequence similarity with the members of the Roseobacter clade of organisms (90–94 %). Other species belonging to the -Proteobacteria examined showed lower levels of similarity. An unrooted tree constructed using the neighbour-joining method shows the phylogenetic position of strain EL-88T among the Proteobacteria, defined by the Roseobacter clade of organisms (Fig. 2). All associations showing bootstrap resampling values of 90 % or more in the neighbour-joining tree were confirmed by parsimony analysis. Analyses demonstrated that strain EL-88T formed a distinct lineage clustering with the genera Jannaschia, Octadecabacter and ‘Marinosulfonomonas’ and the species Ruegeria gelatinovorans. However, EL-88T did not display a particularly close nor statistically significant association (as shown by bootstrap resampling) with any recognized taxa (Fig. 2). Indeed, from sequence divergence (>6 %) and tree topology considerations, strain EL-88T appears to be equivalent in rank to the genera of the Roseobacter clade of organisms. However, it is also evident from the tree-making analyses that the genus Ruegeria, as currently recognized, is interspersed with several other taxa. To fulfil the criteria of being a monophyletic group, the genus Ruegeria (Uchino et al., 1998) should be restricted to the species Ruegeria atlantica and Ruegeria algicola. Note that the phylogenetic distinctiveness of the novel bacterium represented by strain EL-88T is strongly supported by phenotypic considerations. Strain EL-88T is distinguishable from its closest relative, Jannaschia helgolandensis, by its ability to form rosettes, production of bchl a, ability to grow at 5 °C, and utilization of acetate, pyruvate, glutamate and butyrate. Dominant fatty acids of strain EL-88T are 16 : 0 and 18 : 17c, whereas those of J. helgolandensis are methyl 18 : 1, 18 : 17c, 18 : 0 and cyclo 19 : 0, which in lower amounts are also found in Sagittula stellata (Gonzalez et al., 1997) and Antarctobacter heliothermus (Labrenz et al., 1998). Interestingly, neither strain EL-88T nor J. helgolandensis is able to grow with NaCl instead of ASW (Wagner-Döbler et al., 2003). However, from the combination of physiological characteristics, chemotaxonomic and biochemical tests, fatty acid profiles, polar lipid data and 16S rRNA gene sequence analyses it is evident that strain EL-88T represents a hitherto unknown lineage related to, but separate from, the genera Jannaschia, Octadecabacter and ‘Marinosulfonomonas’ and the species Ruegeria gelatinovorans. Therefore, based on both phenotypic and genotypic evidence, we propose that the novel strain EL-88T be classified in a new genus, Roseisalinus gen. nov., as Roseisalinus antarcticus sp. nov.
|
Gram-negative motile rods. Cells contain poly--hydroxybutyrate and do not form spores. The temperature range for growth is <3–33·5 °C. They grow in the presence of 10–130 ASW. Cells have an absolute requirement for Na+ and Cl–, but NaCl cannot replace ASW. pH range for growth is 5·5–9·5. Aerobic to microaerophilic heterotrophs growing on various carboxylic acids and sugars. Cells do not depend on vitamins. No growth on glucose anaerobically in the absence of nitrate. They do not grow photoautotrophically with H2/CO2 (80 : 20) or photo-organotrophically with acetate or glutamate. Cells exhibit peroxidase, catalase and cytochrome oxidase activity. The peptidoglycan contains meso-diaminopimelic acid. Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylcholine are present, but phosphatidylethanolamine and phosphatidylmonomethylamine are not. Dominant cellular fatty acids are 16 : 0 and 18 : 17c, with 3-OH 10 : 0, 16 : 17c and 18 : 0 present in smaller amounts. The respiratory quinone is Q-10. Isolated from water samples from Ekho Lake, Vestfold Hills, East Antarctica.
The type species is Roseisalinus antarcticus.
Description of Roseisalinus antarcticus sp. nov.
Roseisalinus antarcticus (ant.arc'ti.cus. N.L. adj. antarcticus pertaining to the Antarctic).
Cell sizes are in the range 0·90–1·02x2·18–4·20 µm; mean 0·96x3·19 µm. One or both cell poles narrower. Cell growth is monopolar, indicating a budding process. Holdfast structures are often produced and cells have a strong tendency to form rosettes. Colonies on PYGV+ASW are 2 mm in diameter, circular with regular edges, smooth, convex and red. Growth in liquid cultures occurs as small aggregates. Bchl a is produced. Optimal growth occurs at 16–26 °C at concentrations of 50–90 ASW. The optimum pH range is 7·0–7·8. Cells are susceptible to chloramphenicol (30 µg), streptomycin (10 µg), polymyxin B (300 U) and penicillin G (10 U), but not to tetracycline (30 µg). Lipase activity, but DNA, gelatin, starch and alginate are not hydrolysed. Growth occurs on acetate, pyruvate, malate, butyrate, succinic acid, methanesulfonic acid, glutamic acid, cis-aconitic acid, itaconic acid, propionic acid, D-saccharic acid, bromosuccinic acid, Tween 40 (variably on Tween 80), glycerol, glucose, D-arabinose, L-arabinose, ribose, D-xylose, L-xylose, galactose, D-fructose, rhamnose, inositol, mannitol, aesculin, cellobiose, maltose, melibiose, sucrose, -gentiobiose, D-lyxose, D-fucose, L-fucose, D-arabitol and thymidine. Nitrate is assimilatory slightly reduced to nitrite. Cells do not produce acid or acetoin from glucose. No production of sulfide or indole. The DNA G+C content is 67 mol%. Chemotaxonomic properties and other characteristics are as given for the genus.
The type strain is EL-88T (=DSM 11466T=CECT 7023T).
|
ACKNOWLEDGEMENTS |
|---|
|
REFERENCES |
|---|
|
TOPABSTRACTMAIN TEXTREFERENCES |
|---|
Béjà, O., Suzuki, M. T., Heidelberg, J. F., Nelson, W. C., Preston, C. M., Hamada, T., Eisen, J. A., Fraser, C. M. & DeLong, E. F. (2002). Unsuspected diversity among marine aerobic anoxygenic phototrophs. Nature 415, 630–633.[CrossRef][Medline]
Gonzalez, J. M., Mayer, F., Moran, M. A., Hodson, R. E. & Whitman, W. B. (1997). Sagittula stellata gen. nov., sp. nov., a lignin-transforming bacterium from a coastal environment. Int J Syst Bacteriol 47, 773–780.
Gosink, J. J., Herwig, R. P. & Staley, J. T. (1997). Octadecabacter arcticus gen. nov., sp. nov., and O. antarcticus, sp. nov., nonpigmented, psychrophilic gas vacuolate bacteria from polar sea ice and water. Syst Appl Microbiol 20, 356–365.
Harashima, K., Nakagawa, M. & Murata, N. (1982). Photochemical activities of bacteriochlorophyll in aerobically grown cells of aerobic heterotrophs, Erythrobacter species (OCh 114) and Erythrobacter longus (OCh 101). Plant Cell Physiol 23, 185–193.
Holmes, A. J., Kelly, D. P., Baker, S. C., Thompson, A. S., De Marco, P., Kenna, E. M. & Murrell, J. C. (1997). Methylosulfonomonas methylovora gen. nov., sp. nov., and Marinosulfonomonas methylotropha gen. nov., sp. nov.: novel methylotrophs able to grow on methanesulfonic acid. Arch Microbiol 167, 46–53.[CrossRef][Medline]
Labrenz, M. & Hirsch, P. (2001). Physiological diversity and adaptations of aerobic heterotrophic bacteria from different depths of hypersaline, heliothermal and meromictic Ekho Lake (East Antarctica). Polar Biol 24, 320–327.[CrossRef]
Labrenz, M., Collins, M. D., Lawson, P. A., Tindall, B. J., Braker, G. & Hirsch, P. (1998). Antarctobacter heliothermus gen. nov., sp. nov., a budding bacterium from hypersaline and heliothermal Ekho Lake. Int J Syst Bacteriol 48, 1363–1372.
Labrenz, M., Collins, M. D., Lawson, P. A., Tindall, B. J., Schumann, P. & Hirsch, P. (1999). Roseovarius tolerans gen. nov., sp. nov., a budding bacterium with variable bacteriochlorophyll a production from hypersaline Ekho Lake. Int J Syst Bacteriol 49, 137–147.
Labrenz, M., Tindall, B. J., Lawson, P. A., Collins, M. D., Schumann, P. & Hirsch, P. (2000). Staleya guttiformis gen. nov., sp. nov., and Sulfitobacter brevis sp. nov., -3-Proteobacteria from hypersaline, heliothermal and meromictic Antarctic Ekho Lake. Int J Syst Evol Microbiol 50, 303–313.[Abstract]
Labrenz, M., Lawson, P. A., Tindall, B. J., Collins, M. D. & Hirsch, P. (2003). Saccharospirillum impatiens gen. nov., sp. nov., a novel -proteobacterium isolated from hypersaline Ekho Lake (East Antarctica). Int J Syst Evol Microbiol 53, 653–660.
Lafay, B., Ruimy, R., de Traubenberg, C. R., Breittmayer, V., Gauthier, M. J. & Christen, R. (1995). Roseobacter algicola sp. nov., a new marine bacterium isolated from the phycosphere of the toxin-producing dinoflagellate Prorocentrum lima. Int J Syst Bacteriol 45, 290–296.
Lawson, P. A., Collins, M. D., Schumann, P., Tindall, B. J., Hirsch, P. & Labrenz, M. (2000). New LL-diaminopimelic acid-containing actinomycetes from hypersaline, heliothermal and meromictic Antarctic Ekho Lake: Nocardioides aquaticus sp. nov. and Friedmanniella [correction of Friedmannielly] lacustris sp. nov. Syst Appl Microbiol 23, 219–229.[Medline]
Lyman, J. & Fleming, R. H. (1940). Composition of sea water. J Marine Res 3, 134–146.
Mesbah, M., Premachandran, U. & Whitman, W. B. (1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.
Nicholas, K. B., Nicholas, H. B., Jr & Deerfield, D. W., II (1997). GENEDOC: analysis and visualization of genetic variation. EMBNEW News 4, 14.
Page, R. D. M. (1996). TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12, 357–358.
Pearson, W. R. & Lipman, D. J. (1988). Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A 85, 2444–2448.
Pfennig, N. & Wagener, S. (1986). An improved method of preparing wet mounds for photomicrographs of microorganisms. J Microbiol Methods 4, 303–306.[CrossRef]
Rasmussen, S. W. (1995). DNATOOLS: a software package for DNA sequence analysis. Copenhagen: Carlsberg Laboratory.
Rhuland, L. E., Work, E., Denman, R. F. & Hoare, D. S. (1955). The behavior of the isomers of ,
-diaminopimelic acid on paper chromatogramms. J Am Chem Soc 77, 4844–4846.[CrossRef]
Rüger, H. J. & Höfle, M. G. (1992). Marine star-shaped-aggregate-forming bacteria: Agrobacterium atlanticum sp. nov.; Agrobacterium meteori sp. nov.; Agrobacterium ferrugineum sp. nov., nom. rev.; Agrobacterium gelatinovorum sp. nov., nom. rev.; and Agrobacterium stellulatum sp. nov., nom. rev. Int J Syst Bacteriol 42, 133–143.
Saitou, N. & Nei, M. (1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.[Abstract]
Shiba, T. (1991). Roseobacter litoralis gen. nov., sp. nov., and Roseobacter denitrificans sp. nov., aerobic pink-pigmented bacteria which contain bacteriochlorophyll a. Syst Appl Microbiol 14, 140–145.
Sorokin, D. Y. (1995). Sulfitobacter pontiacus gen. nov., sp. nov. – a new heterotrophic bacterium from the Black Sea, specialized on sulfite oxidation. Microbiology (English translation of Mikrobiologiya) 64, 295–305.
Tindall, B. J. (1990a). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13, 128–130.
Tindall, B. J. (1990b). Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 66, 199–202.
Uchino, Y., Hirata, A., Yokota, A. & Sugiyama, J. (1998). Reclassification of marine Agrobacterium species: proposals of Stappia stellulata gen. nov., comb. nov., Stappia aggregata sp. nov., nom. rev., Ruegeria atlantica gen. nov., comb. nov., Ruegeria gelatinovora comb. nov., Ruegeria algicola comb. nov., and Ahrensia kieliense gen. nov., sp. nov., nom. rev. J Gen Appl Microbiol 44, 201–210.
Wagner-Döbler, I., Rheims, H., Felske, A., Pukall, R. & Tindall, B. J. (2003). Jannaschia helgolandensis gen. nov., sp. nov., a novel abundant member of the marine Roseobacter clade from the North Sea. Int J Syst Evol Microbiol 53, 731–738.
This article has been cited by other articles:
|
|
 |
|
  M. Labrenz, P. A. Lawson, B. J. Tindall, and P. Hirsch Roseibaca ekhonensis gen. nov., sp. nov., an alkalitolerant and aerobic bacteriochlorophyll a-producing alphaproteobacterium from hypersaline Ekho Lake Int J Syst Evol Microbiol, August 1, 2009; 59(8): 1935 - 1940. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-X. Wang, Z.-G. Wang, J.-H. Liu, Y.-G. Chen, X.-X. Zhang, M.-L. Wen, L.-H. Xu, Q. Peng, and X.-L. Cui Sediminimonas qiaohouensis gen. nov., sp. nov., a member of the Roseobacter clade in the order Rhodobacterales Int J Syst Evol Microbiol, July 1, 2009; 59(7): 1561 - 1567. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-H. Yoon, S.-J. Kang, J.-S. Lee, and T.-K. Oh Lutimaribacter saemankumensis gen. nov., sp. nov., isolated from a tidal flat of the Yellow Sea Int J Syst Evol Microbiol, January 1, 2009; 59(1): 48 - 52. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z.-P. Liu, B.-J. Wang, X.-Y. Liu, X. Dai, Y.-H. Liu, and S.-J. Liu Paracoccus halophilus sp. nov., isolated from marine sediment of the South China Sea, China, and emended description of genus Paracoccus Davis 1969 Int J Syst Evol Microbiol, January 1, 2008; 58(1): 257 - 261. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-Y. Ying, B.-J. Wang, X. Dai, S.-S. Yang, S.-J. Liu, and Z.-P. Liu Wenxinia marina gen. nov., sp. nov., a novel member of the Roseobacter clade isolated from oilfield sediments of the South China Sea Int J Syst Evol Microbiol, August 1, 2007; 57(8): 1711 - 1716. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. H. Choi and B. C. Cho Citreimonas salinaria gen. nov., sp. nov., a member of the Roseobacter clade isolated from a solar saltern Int J Syst Evol Microbiol, December 1, 2006; 56(12): 2799 - 2803. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Eiler Evidence for the Ubiquity of Mixotrophic Bacteria in the Upper Ocean: Implications and Consequences Appl. Envir. Microbiol., December 1, 2006; 72(12): 7431 - 7437. [Full Text] [PDF]
|
|
|
|
|
|
T. Martens, T. Heidorn, R. Pukall, M. Simon, B. J. Tindall, and T. Brinkhoff Reclassification of Roseobacter gallaeciensis Ruiz-Ponte et al. 1998 as Phaeobacter gallaeciensis gen. nov., comb. nov., description of Phaeobacter inhibens sp. nov., reclassification of Ruegeria algicola (Lafay et al. 1995) Uchino et al. 1999 as Marinovum algicola gen. nov., comb. nov., and emended descriptions of the genera Roseobacter, Ruegeria and Leisingera Int J Syst Evol Microbiol, June 1, 2006; 56(6): 1293 - 1304. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. B. M. Denner, M. Kolari, D. Hoornstra, I. Tsitko, P. Kampfer, H.-J. Busse, and M. Salkinoja-Salonen Rubellimicrobium thermophilum gen. nov., sp. nov., a red-pigmented, moderately thermophilic bacterium isolated from coloured slime deposits in paper machines Int J Syst Evol Microbiol, June 1, 2006; 56(6): 1355 - 1362. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Gich and J. Overmann Sandarakinorhabdus limnophila gen. nov., sp. nov., a novel bacteriochlorophyll a-containing, obligately aerobic bacterium isolated from freshwater lakes. Int J Syst Evol Microbiol, April 1, 2006; 56(Pt 4): 847 - 854. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Dai, B.-J. Wang, Q.-X. Yang, N.-Z. Jiao, and S.-J. Liu Yangia pacifica gen. nov., sp. nov., a novel member of the Roseobacter clade from coastal sediment of the East China Sea. Int J Syst Evol Microbiol, March 1, 2006; 56(Pt 3): 529 - 533. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. R. Arahal, M. C. Macian, E. Garay, and M. J. Pujalte Thalassobius mediterraneus gen. nov., sp. nov., and reclassification of Ruegeria gelatinovorans as Thalassobius gelatinovorus comb. nov. Int J Syst Evol Microbiol, November 1, 2005; 55(6): 2371 - 2376. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Pujalte, M. C. Macian, D. R. Arahal, and E. Garay Thalassobacter stenotrophicus Macian et al. 2005 is a later synonym of Jannaschia cystaugens Adachi et al. 2004, with emended description of the genus Thalassobacter Int J Syst Evol Microbiol, September 1, 2005; 55(5): 1959 - 1963. [Abstract] [Full Text] [PDF]
|
|
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
