Burkholderia tropica sp. nov., a novel nitrogen-fixing, plant-associated bacterium, by V. M. Reis, P. Estrada-de los Santos, S. Tenorio-Salgado, J. Vogel, M. Stoffels, S. Guyon, P. Mavingui, V. L. D. Baldani, M. Schmid, J. I. Baldani, J. Balandreau, A. Hartmann and J. Caballero-Mellado
International Journal of Systematic and Evolutionary Microbiology vol. 54, part 6, pp. 2155 - 2162
Supplementary Fig. A. ARDRA profiles of B. tropica strains. Lanes: 1 and 6, 100 base pair molecular marker (Gibco BRL). Lanes 2 to 5, 16S rRNA amplified gene digested with DdeI; lane 2, strain MOc-725; lane 3, strain MTo-672; lane 4, strain MTo-293; lane 5, strain Ppe8T. Lanes 7 to 10, 16S rRNA amplified gene digested with HinfI; lane 7, strain MOc-725; lane 8, strain MTo-672 (genotype 17); lane 9, strain MTo-293; lane 10, strain Ppe8T.
Supplementary Fig. B. Extended phylogenetic tree based on 16S rRNA gene sequences showing the relatedness among Burkholderia tropica sp. nov., Burkholderia species and related beta-Proteobacteria. The bar represents 1 nucleotide substitution per 100 nucleotides. Bootstrap probabilities are indicated at branch points. The GenBank accession number for each strain is shown in parentheses.
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Supplementary material. BIOLOG carbon source utilization tests by B. tropica sp. nov.
According to the BIOLOG carbon source utilization (oxidation) tests, all B. tropica strains were able to oxidize Tween 40, Tween 80, N-acetyl-D-glucosamine, adonitol, L-arabinose, D-arabitol, cellobiose, D-fructose, L-fucose, D-galactose, alpha-D-glucose, D-mannitol, mannose, L-rhamnose, methyl pyruvate, monomethyl succinate, cis-aconitic acid, citric acid, D-gluconic acid, beta-hydroxybutyric acid, p-hydroxyphenylacetic acid, DL-lactic acid, propionic acid, quinic acid, sebacic acid, succinic acid, bromosuccinic acid, L-alanylglycine, L-asparagine, L-aspartic acid, L-glutamic acid, hydroxy-L-proline, L-phenylalanine, L-proline, gamma-aminobutyric acid and 2-aminoethanol. The strains were unable to oxidize alpha-cyclodextrin, dextrin, glycogen, N-acetyl-D-galactosamine, erythritol, alpha-D-lactose, maltose, melibiose, methyl beta-D-glucoside, D-raffinose, sucrose, turanose, xylitol, D-galactonic acid lactone, D-glucuronic acid, gamma-hydroxybutyric acid, alpha-ketovaleric acid, malonic acid, glucoronamide, glycyl-L-aspartic acid, D-serine, urocanic acid, uridine, phenylethylamine and glucose 1-phosphate. Variable results were obtained for the oxidation of D-sorbitol, formic acid, D-galacturonic acid, D-saccharic acid, L-serine, alaninamide, L-pyroglutamic acid, inosine, myo-inositol, acetic acid, D-glucosaminic acid, alpha-hydroxybutyric acid, DL-alanine, L-histidine, gentiobiose, lactulose, D-psicose, itaconic acid, L-ornithine, DL-carnitine, putrescine, alpha-ketobutyric acid, alpha-ketoglutaric acid, succinamic acid, L-threonine, glucose 6-phosphate, D-trehalose, glycyl-L-glutamic acid, 2,3-butanediol, glycerol, glycerol phosphate and L-leucine.
