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Mycobacterium parascrofulaceum sp. nov., novel slowly growing, scotochromogenic clinical isolates related to Mycobacterium simiae, by C. Y. Turenne, V. J. Cook, T. V. Burdz, R. J. Pauls, L. Thibert, J. N. Wolfe and A. Kabani

International Journal of Systematic and Evolutionary Microbiology vol. 54, part 5, pp. 1543 - 1551

Supplementary Fig. A. Alignment of the 16S-23S rRNA spacer (ITS1) sequences. SqvI-SqvIV, M. parascrofulaceum sp. nov. sequevars; Mscrof, M. scrofulaceum ATCC 19981T (determined in our laboratory). Nucleotides that differ from M. scrofulaceum are shown. The length (in nt) of the ITS1 fragment from position 1 is indicated.

Supplementary Fig. B. Alignment of a hypervariable region of the hsp65 gene. Numbering of Mtb represents that of the complete hsp65 gene using the sequence M. tuberculosis Erdman 65 kDa (M15467). All other sequences were determined in our laboratory. Numbering of all other sequences represents the base position on the amplified 441 bp fragment. SqvI-SqvV, M. parascrofulaceum sp. nov. sequevars; Msc, M. scrofulaceum ATCC 19981T; Mav, M. avium ATCC 25291T; Min, M. intracellulare ATCC 13950T.

Supplementary Table A. 16S rRNA gene sequence analysis of M. parascrofulaceum sp. nov. strain HSC-68T against its genotypically closest relatives.

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