Classification of phytoplasma strains in the elm yellows group (16SrV) and proposal of 'Candidatus Phytoplasma ulmi' for the phytoplasma associated with elm yellows, by I.-M. Lee, M. Martini, C. Marcone and S. F. Zhu
International Journal of Systematic and Evolutionary Microbiology vol. 54, part 2, pp. 337 - 347
Supplementary Table A. Summary of pattern groups produced by RFLP analyses of 16S rDNA from representative strains in the elm yellows phytoplasma group
Supplementary Table B. Summary of pattern groups produced by RFLP analyses of ribosomal protein gene sequences from representative strains in the elm yellows phytoplasma group
[PDF of Tables A and B] (121 KB)
Supplementary Fig. A. Analysis of putative restriction sites in ribosomal protein operon sequences (1.2 kb, contains rpl22 and rps3 genes), amplified by nested PCR with primer pair rp(V)F1/rpR1 followed by primer pair rp(V)F1A/rp(V)R1A from representative phytoplasma strains in the elm yellows phytoplasma group (16SrV). Maps were generated using the MapDraw option of the DNASTAR program and aligned manually for comparison of restriction sites. Asterisks indicate putative restriction sites that differentiate the representative strains in the elm yellows phytoplasma group (16SrV). Strain abbreviations are defined in Table 1. [PDF] (115 KB)
Supplementary Fig. B. Analysis of putative restriction sites in the secY gene sequence (1.2 kb). The sequence was trimmed from a DNA fragment (1.4 kb) amplified by PCR with primer pair FD9f/r from representative phytoplasma strains in the elm yellows phytoplasma group (16SrV). Maps were generated using the MapDraw option of the DNASTAR program and aligned manually for comparison of restriction sites. Asterisks indicate putative restriction sites that differentiate the representative strains in the elm yellows phytoplasma group (16SrV). Strain abbreviations are defined in Table 1. [PDF] (111 KB)
