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Herbaspirillum lusitanum sp. nov., a novel nitrogen-fixing bacterium associated with root nodules of Phaseolus vulgaris, by A. Valverde, E. Velázquez, C. Gutiérrez, E. Cervantes, A. Ventosa & J.-M. Igual

International Journal of Systematic and Evolutionary Microbiology vol. 53, part 6, pp. 1979 - 1983.

Supplementary Table A. DNA G+C contents and levels of DNA–DNA relatedness of strain P6-12^T to other related strains and Herbaspirillum species

Supplementary Table B. Pellicle formation in semi-solid media by Herbaspirillum type strains with different carbon sources

[PDF of Supplementary Tables A and B] (122 KB)

Supplementary Fig. A. Transmission electron micrographs of strain P6-12^T grown in nutrient agar for 48 h showing one (a) and two (b) polar flagella. Bars, 0.15 µm.

Supplementary Fig. B. Phylogenetic analysis based on 16S rRNA gene sequences available from GenBank (accession numbers in parentheses) constructed after multiple alignment of data by CLUSTAL W software, showing the position of strain P6-12^T. Distances were calculated according to Kimura's two-parameter method and clustering with neighbour-joining method was performed by using the software package MEGA 2.1, as described in Methods. Bootstrap values based on 1000 replications are listed as percentages at branching points. The tree was rooted using Comamonas testosteroni ATCC 11996^T as the outgroup. Bar, 1 substitution per 100 nt. [PDF] (105 KB)

Supplementary Fig. C. RFLP patterns after digestion with the restriction endonucleases DdeI and CfoI (Amersham-Pharmacia Biotech) of 16SrDNA of the type strains of previously described species of Herbaspirillum and of strains isolated in this study (represented by strain P6-12^T). Lanes: 1 and 5, H. seropedicae DSM 6445^T; 2 and 6, H. rubrisubalbicans DSM 9440^T; 3 and 7, H. frisingense DSM 13128^T; 4 and 8, strain P6-12^T; MW, 100 bp ladder (Pharmacia).

Supplementary Fig. D. TP-RAPD patterns of the type strains of previously described species of Herbaspirillum and of strains isolated in this study (represented by strain P6-12^T). Lanes: 1, H. seropedicae DSM 6445^T; 2, H. rubrisubalbicans DSM 9440^T; 3, H. frisingense DSM 13128^T; 4, strain P6-12^T; MW, MassRuler molecular mass marker (Fermentas).

This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Citing Articles Citing Articles via CrossRef