Hanseniaspora meyeri sp. nov., Hanseniaspora clermontiae sp. nov., Hanseniaspora lachancei sp. nov. and Hanseniaspora opuntiae sp. nov., novel apiculate yeast species, by N. Cadez, G. A. Poot, P. Raspor & M. Th. Smith
International Journal of Systematic and Evolutionary Microbiology vol. 53, part 5, pp. 1671 - 1680
Supplementary Table. DNA–DNA relatedness among some strains of the genera Hanseniaspora and Kloeckera. [PDF] (125 KB)
Supplementary Fig. A. Phylogenetic positions of four novel species of Hanseniaspora and CBS 8772 based on the D1/D2 region of 26S rDNA. The tree was constructed by neighbour-joining analysis. Bootstrap percentages above 50 % from 1000 bootstrap replicates are shown. Names in bold correspond to sequences determined in this study. The outgroup species was Saccharomyces cerevisiae. Bar, 1 % sequence divergence. [PDF] (243 KB)
Supplementary Fig. B. Molecular identification scheme for separation of physiologically indistinguishable Hanseniaspora and Kloeckera species by PCR-RFLP of ITS regions. Restriction enzyme MboII separates H. uvarum from H. meyeri or H. opuntiae from H. guilliermondii; HinfI separates CBS 8772 from H. guilliermondii and H. opuntiae or H. valbyensis from K. lindneri; and DdeI separates H. vineae from H. osmophila. Sizes of restriction fragments are shown. Numbers in bold indicate double bands. [PDF] (838 KB)
