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1 Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China
2 Department of Microbiology, College of Life Science, Nankai University, Tianjin 300071, China
Correspondence
Yanhe Ma
mayh{at}sun.im.ac.cn
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ABSTRACT |
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The GenBank/EMBL/DDBJ accession number for the 16S rDNA sequence of strain HD321T is AF432348.
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). The species of Gordonia show considerable metabolic diversity and are capable of degrading toxic environment-contaminating compounds, as exemplified by the isolation of strains possessing hydrocarbon-oxidizing, rubber-degrading, aromatic-desulphurizing and 3-ethylpyridine-degrading pathways (Kummer et al., 1999; Linos et al., 2002; Kim et al., 1999; Gilbert et al., 1998; Rhee et al., 1998; Yoon et al., 2000). From the point of view of bioremediation, the genus Gordonia is a very important taxon. Some species have been used for decontamination of polluted soils and waters (Bell et al., 1998). The members of the genus Gordonia are widely distributed in various environments such as soil, activated sludge, biofilm and the mangrove rhizosphere (Tsukamura, 1971; Lechevalier & Lechevalier, 1974; Bendinger et al., 1995; Takeuchi & Hatano, 1998; Linos et al., 1999; Kummer et al., 1999). There are no reports of Gordonia species in the producing-well water of oilfields, which has low levels of nutrients, consisting mainly of straight-chain and aromatic hydrocarbons.
In the course of an investigation of the microflora in the Daqing oilfield formation, we isolated several hydrocarbon-degrading strains from well-bore water samples (Nazina et al., 2000). A hydrocarbon-degrading bacterium, designated strain HD321T, appeared to represent a novel taxon based on preliminary investigations of its phenotypic and phylogenetic characteristics. Here, we describe the physiological, chemotaxonomic and phylogenetic characteristics of strain HD321T and propose a novel species of the genus Gordonia, Gordonia paraffinivorans sp. nov., for this strain.
Strain HD321T was isolated from a water sample collected in late May 1996 from a producing well of Daqing oilfield, China. The water sample was inoculated into 100 ml minimal salts medium containing (l-1) 1·0 g NH4Cl, 0·5 g KH2PO4, 0·75 g Na2HPO4.12H2O, 0·2 g MgSO2.7H2O, 0·02 g CaCl2 and 10 ml trace-element solution (Lee et al., 1991), supplemented with 5 ml liquid paraffin. This medium was incubated at 37 °C on a rotary shaker at 250 r.p.m. After the liquid paraffin was emulsified by growth of micro-organisms, a portion of the cultivation medium was diluted and plated on nutrient agar containing (l-1) 10 g tryptone, 3 g beef extract, 5 g NaCl and 15 g agar. Strain HD321T was isolated on nutrient agar and showed orange-red colonies on glucose potato agar (GPA).
Cell morphology was determined by phase-contrast and electron microscopy. Mobility was determined by the hanging-drop method. The optimal conditions for growth were determined in nutrient broth with 0–14 % NaCl, pH 5–10 and at 4–60 °C. Carbon source utilization was tested in the medium described previously (Kämpfer et al., 1990). Controls were grown without substrate addition. The ability to grow in the presence of 0·1 % oleic acid and 0·001 % zinc chloride was tested as described previously (Kim et al., 1999). The methods used for biochemical tests were described previously (Smibert & Krieg, 1981). The isomeric form of diaminopimelic acid in the cell wall was analysed using TLC according to the method of Komagata & Suzuki (1987). The sugars of the cell wall were analysed as described previously (Saddler et al., 1991). Menaquinones were extracted and purified from freeze-dried cells using the method of Collins (1985) and determined by reversed-phase HPLC. Fatty acid analysis was performed using standard methods and compared to the database of fatty acids in the MIDI Sherlock Microbial Identification System (Microbial ID). Mycolic acid was determined by the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ, Braunschweig, Germany). Genomic DNA of the strain was prepared by the method of Marmur (1961) and the purity was checked spectrometrically. The G+C content of the DNA was determined by the thermal denaturation method (Marmur & Doty, 1962). The methods used for PCR amplification of 16S rRNA gene, sequencing of the PCR products and determination of the phylogenetic position were described previously (Zhang et al., 2002). The reference strains used in sequence comparison are shown in Fig. 1.
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). The strain was a Gram-positive, non-acid-fast, non-motile, rod-shaped and strictly aerobic bacterium. Cells (0·3–0·5x1–2 µm) often occurred singly or in a typical coryneform V-shape. Catalase and urease were positive. Oxidase was negative. Gelatin and cellulose were not hydrolysed. Strain HD321T grew well at 30–37 °C and very slowly at 20 and 45 °C. Growth occurred in medium containing 0·5–7 % (w/v) NaCl and at pH 5·5–9·5. Some other biochemical properties useful for identifying and differentiating strain HD321T and type strains of other species of the genus Gordonia are represented in Table 1 and in the description of the species.
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; Takeuchi & Hatano, 1998; Stackebrandt et al., 1988). The cell wall of strain HD 321T contained meso-diaminopimelic acid as the only diamino acid and arabinose and galactose as the major cell-wall sugars, indicating that the wall chemotype was type IV (Lechevalier & Lechevalier, 1970). The predominant menaquinone was MK-9(H2). The fatty acid profile of strain HD321T was composed of C16 : 0 (25·8 %), tuberculostearic acid (10-methyl-C18 : 0) (22·4 %), C18 : 1
9c (20·5 %), summed feature 4 (C16 : 17c and/or i-C15 : 0 2-OH) (11·5 %), C18 : 0 (9·3 %), C14 : 0 (2·7 %), C17 : 0 (2·2 %), C17 : 18c (1·6 %) and C20 : 0 (1·2 %). Mycolic acids ranged from 52 to 62 carbon atoms (2·8 % C52, 6·7 % C54, 25·2 % C56, 2·5 % C57, 41 % C58, 1 % C59, 19·1 % C60, 1·7 % C62), with C56 and C58 being the principal mycolic acids. The G+C content of the DNA was 66·0 mol%. Fine qualitative and quantitative differences in fatty acid patterns and mycolic acid chain lengths could distinguish strain HD321T from type strains of other species of the genus Gordonia.
The 16S rDNA of strain HD321T was amplified by PCR and an almost-complete nucleotide sequence (1472 bp) was determined by direct sequencing. The phylogenetic tree showed that strain HD321T was in the cluster comprising members of the genus Gordonia and formed a monophyletic clade (Fig. 1
). The 16S rDNA sequence similarities between strain HD321T and the type strains of other Gordonia species with validly published names were 95·3–97·6 %. The highest level of sequence similarity (97·6 %) was to Gordonia amicalis and Gordonia rubripertincta. These values and the results shown in the phylogenetic tree indicate that strain HD321T represents a novel member of the genus Gordonia (Stackebrandt & Goebel, 1994). Because of the ability of the strain to use paraffin as a carbon source, the name Gordonia paraffinivorans sp. nov. is proposed.
Description of Gordonia paraffinivorans sp. nov.
Gordonia paraffinivorans (pa.raf.fi.ni.vo'rans. N.L. n. paraffina paraffin; L. part. adj. vorans devouring; N.L. part. adj. paraffinivorans paraffin-devouring, referring to the ability to degrade paraffin).
Aerobic, Gram-positive, non-motile, short rod-shaped bacterium (0·3–0·5x1–2 µm); orange-red colonies formed on GPA. No spore formation is observed. Oxidase-negative. Catalase- and urease-positive. Sucrose, maltose, glucose, D-fructose, D-galactose, D-mannose, inulin, dextrin, L-proline, L-alanine, L-leucine, glutamate and glycerol are used as sole sources of carbon for energy and growth. Does not utilize L-arabinose, D-raffinose, L-rhamnose, D-ribose, lactose, D-cellobiose, L-sorbose, D-xylose, sorbitol, D-mannitol, D-arabitol, aesculin, L-malic acid, succinic acid, L-lysine, oxalate, citrate or tartrate. Acids are not produced from glucose, sucrose, D-mannose, D-galactose or glycerol. Hypoxanthine, xanthine, tyrosine, starch, arbutin, uric acid, gelatin, cellulose and Tween 20 are not degraded. Aesculin, allantoin and Tween 80 are hydrolysed. Growth occurs in the presence of oleic acid (0·8 %, w/v) and zinc chloride (0·001 %, w/v). Positive for formation of H2S; negative for production of indole and methyl red test. Nitrate is not reduced to nitrite. Cell wall contains meso-diaminopimelic acid, arabinose and galactose (cell-wall chemotype IV sensu Lechevalier & Lechevalier, 1970). The predominant menaquinone is MK-9(H2). Mycolic acids have 52–62 carbon atoms, with C56 and C58 being the principal mycolic acids. The major fatty acids are C16 : 0, C18 : 19c and 10-methyl-C18 : 0 (tuberculostearic acid). The G+C content of the DNA of the type strain is 66 mol%.
The type strain, strain HD321T (=AS 4.1730T=DSM 44604T), was isolated from a producing-well water sample from Daqing oilfield, China.
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ACKNOWLEDGEMENTS |
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