Supplementary zymogram
Proposal of Ureaplasma parvum sp. nov. and emended description of Ureaplasma urealyticum (Shepard et al. 1974) Robertson et al. 2001 , by J. A. Robertson, G. W. Stemke, J. W. Davis Jr, R. Harasawa, D. Thirkell, F. Kong, M. C. Shepard and D. K. Ford
International Journal of Systematic and Evolutionary Microbiology vol. 52, part 2, pp. 587-597
Zymogram of inorganic pyrophosphatase (PPase) activity from protein extracts of the serovar standard strains of ureaplasmas from humans after electrophoretic separation on a vertical slab polyacrylamide gel. The assay (Davis et al., 1987) indentified the site of phosphatase release after sodium pyrophosphate was supplied as substrate. Lanes 1–14 are cell-free extracts (15 micrograms protein) of the respective serovars; the PPase of cluster A strains (T960T biovar) had a smaller molecular weight than those from cluster B strains (parvo biovar). The lane designated 'M' contained 30 micrograms concentrated culture-medium components (15 micrograms protein) as a negative control, whilst the lane designated 'Pc' contained yeast PPase as a positive control (0.15 U). The unmarked lane on the extreme right contained the negative control (Davis & Villaneuva, 1990). (John W. Davis, Jr, unpublished photograph.)
