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Bacillus rigui sp. nov., isolated from wetland freshwater

¹ Sunchon National University;
² Gwangju Health College University;
³ KRIBB

⁴ E-mail: scnu{at}scnu.ac.kr

Two Gram-positive strains, WPCB074T and WPCB165, were isolated from fresh water collected from the Woopo wetland (Republic of Korea). Both strains are strictly aerobic, motile and endospore-forming rods. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strains WPCB074T and WPCB165 belonged to the genus Bacillus, and that strain WPCB074T was most closely related to Bacillus solisalsi YC1T (98.4 % sequence similarity), Bacillus barbaricus V2-BIII-A2T (97.7 %), Bacillus macauensis ZFHKFT (96.9 %), Bacillus arsenicus Con a/3T (96.4 %) and Bacillus gelatini LMG 21880T (95.1 %). Between the 16S rRNA gene sequences of strains WPCB074T and WPCB165 there was one nucleotide difference (99.9 %) and suggested that these two strains constituted a single species. DNA-DNA relatedness between strain WPCB074T and the type strains of Bacillus solisalsi, B. barbaricus, B. macauensis, B. arsenicus and B. gelatini were 26, 17, 20, 14 and 7 %, respectively. Strain WPCB074T was characterized by having cell wall peptidoglycan based on meso-diaminopimelic acid, MK-7 as the predominant menaquinone, iso-C15:0 and anteiso-C15:0 as the major fatty acids. The G+C content of strain WPCB074T is 41.9 mol%. On the basis of phenotypic properties, phylogeny and genomic distinctiveness, strain WPCB074T represent a new species of the genus Bacillus, for which a new name, Bacillus rigui is proposed. The type strain of the new species is strain WPCB074T (= KCTC 13278T = JCM 16348T).