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1 China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan;
2 College of Life Science and Technology, Xinjiang University, Urumchi 830046, China
3 E-mail: cxfang{at}whu.edu.cn
A novel strain, HY-22RT, was isolated from soil of a Euphrate poplar forest in Xinjiang, China. The cells were Gram-positive, rod-shaped and motile by means of peritrichous flagella. Growth occurred at 10-37°C (optimum, 30°C), at pH 7.0-8.0 (optimum, pH 7.0) and with 0-1 % NaCl. A phylogenetic analysis based on 16S rRNA gene sequences revealed that strain HY-22RT was closely related to Cohnella phaseoli GSPC1T (96.3 % sequence similarity). The major respiratory quinone was MK-7 and the predominant fatty acids were anteiso-C15:0, iso-C15:0, iso-C16:0 and C16:0. Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content was 49.6mol%. On the basis of the phylogenetic, physiological and chemotaxonomic data, strain HY-22RT represents a novel species in the genus Cohnella, for which the name Cohnella luojiensis sp. nov. is proposed. The type strain is HY-22RT (= CCTCC AB 208254T = NRRL B-59213T).
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