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Lentibacillus persicus sp. nov., a moderately halophilic species isolated from a saline lake

¹ University of Sevilla, Spain;
² University of Tehran, Iran

³ E-mail: ventosa{at}us.es

A Gram-positive, moderately halophilic bacterium, designated strain Amb31T, was isolated from water of the hypersaline lake Aran-Bidgol in Iran and it was taxonomically characterized using a polyphasic approach. Cells were rod-shaped, motile and able to produce ellipsoidal endospores at a central position in swollen sporangia. It was facultatively anaerobic, catalase and oxidase positive. Isolate Amb31T grew in a complex medium supplemented with 3-25% (w/v) NaCl, with the optimum at 7.5-10% (w/v) NaCl. The optimal growth was at 30-35°C and pH 7.5. Phylogenetic analysis based on 16S rRNA gene sequence comparisons showed that strain Amb31T belonged to the genus Lentibacillus; it exhibited 16S rRNA gene sequence similarity values of 96.8, and 96.4% to the type strain of Lentibacillus salicampi and Lentibacillus salinarum, respectively and values of 95.9-94.7% to the type strains of other recognized species of Lentibacillus. Strain Amb31T had cell wall peptidoglycan based on meso-diaminopimelic acid and MK-7 as the respiratory isoprenoid quinone. The major fatty acids were anteiso-C15:0 (44.7 %), iso-C16:0 (21.4 %) and anteiso-C17:0 (15.9 %) and its polar lipid pattern consisted of phosphatidylglycerol, diphosphatidylglycerol, five phospholipids and a glycolipid. The DNA G+C content was 44.1 mol%. All those features confirmed the placement of isolate Amb31T within the genus Lentibacillus; it could be clearly differentiated from other species of Lentibacillus on the basis of several phenotypic, genotypic and chemotaxonomic features. The DNA-DNA hybridization with the most closely related species, Lentibacillus salicampi DSM 16425T was 28%. Therefore, strain Amb31T represents a novel species of the genus Lentibacillus, for which the name Lentibacilus persicus sp. nov. is proposed. The type strain is Amb31T (=CCM 7683T = CECT 7524T = DSM 22530T = LMG 25304T).