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Sporosarcina contaminans sp. nov., and Sporosarcina thermotolerans sp. nov., two novel endospore-forming species

¹ Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen;
² Culture Collection University Göteborg, Dept. of Clinical Bacteriology, S-41346 Göteborg;
³ Deutsche Sammlung von Mikroorganismen und Zellkulturen, D-38124 Braunschweig, Germany

⁴ E-mail: peter.kaempfer{at}umwelt.uni-giessen.de

Two Gram-positive endospore-forming rods (CCUG 53915T and CCUG 53480T) were isolated from an industrial clean room floor and from a human blood sample and were studied for their taxonomic position. By 16S rRNA gene sequence similarity studies, both isolates clearly clustered with Sporosarcina species. Strain CCUG 53915T was most closely related to Sporosarcina koreensis and Sporosarcina soli showing 99.4% and 99.2% gene sequence similarities to these species, respectively. Strain CCUG 53480T showed the highest 16S rRNA gene sequence similarity to S. koreensis (98.7%) and Sporosarcina saromensis (98.6%). Strains CCUG 53915T and CCUG 53480T show the peptidoglycan type A4 L-Lys-D-Glu. The quinone systems of both strains is composed of menaquinone MK-7 and lower amounts of MK-8. The polar lipid profile of the strains consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and three unidentified phospholipids. The fatty acids profiles comprising anteiso- iso- branched fatty acids supported the affiliation of the two isolates to the genus Sporosarcina. The results of physiological and biochemical tests and DNA-DNA hybridizations allowed a clear phenotypic and genotypic differentiation of both strains from the most closely related Sporosarcina species. For this reason, we propose for strain CCUG 53915T (=DSM 22204T) a new species with the name Sporosarcina contaminans sp. nov., and for strain CCUG 53480T (= DSM 22203T) a second new species with the name Sporosarcina thermotolerans sp. nov.