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Caldanaerobacter uzonensis sp. nov., an anaerobic, thermophilic, heterotrophic bacterium isolated from hot springs

¹ Winogradskiy INMI RAS;
² Bioengineering Center, RAS

³ E-mail: kublanov.ilya{at}gmail.com

An anaerobic thermophilic bacterium, strain K67T, was isolated from a terrestrial hot spring of Uzon Caldera, Kamchatka Peninsula. Analysis of 16S rRNA gene sequence revealed that the novel isolate belongs to the genus Caldanaerobacter, with 95 % 16S rRNA identity with the type strain Caldanaerobacter subterraneus subsp. subterraneus, thus representing a novel species of the genus Caldanaerobacter. The novel isolate was characterized as an obligate anaerobe, thermophile, growing at temperatures from 50 to 75°C with the optimum at 68-70°C, neutrophile, growing in the pH^{25 °C} range from 4.8 to 8.0, with the optimum at 6.8, and an obligate organotroph, growing by fermentation of various sugars, peptides and polysaccharides. Major fermentation products were acetate, H2 and CO2; ethanol, lactate and L-alanine were formed in less amount. Thiosulfate stimulated the growth and was reduced to hydrogen sulfide. Nitrate, sulfate, sulfite and elemental sulfur were not reduced and did not stimulate the growth. Thus, according to phylogenetic position and phenotypic novelties (lower temperature characteristics of the growth, the ability to grow on arabinose, the inability to reduce elemental sulfur in the course of growth, formation of alanine as a minor fermentation product), a novel species, Caldanaerobacter uzonensis, sp. nov. is proposed, with the type strain K67T, (DSM 18923T and VKM B-2408T).