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Piscibacillus halophilus sp. nov., a moderately halophilic bacterium from a hypersaline lake

¹ University of Tehran;
² University of Sevilla;
³ Department of Biology, Islamic Azad University, Science and Research Campus, Tehran, Iran

⁴ E-mail: ventosa{at}us.es

A Gram-positive, moderately halophilic bacterium, designated strain HS224T, was isolated from the hypersaline lake Howz-Soltan in Iran. The strain was rod-shaped, nonmotile and produced oval endospores. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain HS224T is phylogenetically affiliated to the genus Piscibacillus, exhibiting a sequence similarity of 98.5% with respect to Piscibacillus salipiscarius; the other closely related species were Aquisalibacillus elongatus (98.0%), Filobacillus milosensis (97.9%) and Tenuibacillus multivorans (97.0%). Strain HS224T was able to grow at NaCl concentrations from 1 to 20% (w/v), with optimum growth occurring at 10% (w/v) NaCl. The optimum temperature and pH for growth were 35 °C and pH 7.5. Similarly to P. salipiscarius the major polar lipids were phosphatidylglycerol and diphosphatidylglycerol, the isoprenoid quinone was MK-7 and the peptidoglycan type was A1, with meso-diaminopimelic acid as the diagnostic diamino acid. The major cellular fatty acids were anteiso-C15:0, iso-C15:0, anteiso-C17:0 and iso-C16:0. The G+C content of the DNA was 37.5 mol%. The DNA-DNA relatedness of the strain with respect to Piscibacillus salipiscarius JCM 13188T was 30.8%. It is evident from the genotypic, chemotaxonomic and phenotypc data that strain HS224T represents a new species of the genus Piscibacillus, for which the name Piscibacillus halophilus sp. nov. is proposed. The type strain is HS224T (=CCM 7596T =DSM 21633T =JCM 15721T =LMG 24786T).