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1 University of Science and Technology;
2 Ewha Womans University;
3 Kyung Hee University
4 E-mail: baejw{at}khu.ac.kr
Strain BL06T was isolated from landfill soil in Pohang, Korea. Strain BL06T is gram-negative, aerobic, and requires 0-6 % NaCl for growth. The temperature range for growth is 10-44 °C, and the pH range is 5.5-12.0. Based on the 16S rRNA gene and gyrase B (gyrB) gene sequences, phylogenetic analysis showed that strain BL06T is associated with the genus Alishewanella and closely related type strains (98.8 % with Alishewanella. aestuarii; 98.7 % with A. fetalis; 98.5 % with A. jeotgali). Physiological and biochemical tests verified that strain BL06T is genotypically and phenotypically different from reference strains in the genus Alishewanella. DNA-DNA hybridization experiments showed that relatedness between the genomic DNA of strain BL06T and other species is less than 41 %. These findings strongly suggest that the strain is a novel species despite high similarity between the 16S rRNA gene sequence of strain BL06T and the reference strains. Therefore, strain BL06T (= KCTC 22400T = JCM 15597T) is proposed to be a novel species named Alishewanella agri sp. nov.
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