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Staphylococcus massiliensis sp. nov., isolated from a human brain abscess sample

Laboratoire de Bactériologie, Hôpital de la Timone, Marseille, France

¹ E-mail: vroux91{at}hotmail.com

Gram positive cocci catalase-positive, coagulase-negative, non-motile, non-fermentative and novobiocin-susceptible were isolated from a human brain abscess sample (strain 5402776T). This strain was analysed by a polyphasic taxonomic approach. The found respiratory quinones were MK-7 (93%) and MK-6 (7%), and the major fatty acids were C15:0 iso (60.5%), C17:0 iso (8.96%) C15:0 anteiso (7.93%) and C19:0 iso (6.78%). The peptidoglycan type found was A3 L-Lys-Gly_2-3-L-Ser-Gly. Based on cellular morphology and biochemical criteria the isolate was assigned to the genus Staphylococcus, although it did not correspond to any recognized species. The G+C content of DNA was 36.6%. Phylogenetic analysis based on 16S rRNA gene sequence comparison, showed that the isolate was most closely related to S. piscifermentans, S. condimenti, S. carnosus subsp. carnosus, S. carnosus subsp. utilis and S. simulans (97.7%, 97.6%, 97.6%, 97.6% and 96.5% of similarity, respectively). Comparison of tuf, hsp60, rpoB, dnaJ and sodA gene sequences was also performed. In phylogenetic analysis inferred from tuf, dnaJ and rpoB gene sequence comparisons strain 5402776T clustered with S. pettenkoferi (93.7%, 82.5% and 89% of similarity, respectively) and on phylogenetic analysis inferred from sodA gene sequence comparison it clustered with S. chromogenes (82.8%). On the basis of phenotypic and genotypic data, we propose that this isolate represents a novel species Staphylococcus massiliensis sp. nov. (type strain 5402776T=CCUG 55927T=CSUR P23T).