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Kineococcus rhizosphaerae sp. nov., isolated from rhizosphere soil

Department of Science Education, Cheju National University, Jeju 690-756, Republic of Korea

Correspondence
Soon Dong Lee
sdlee{at}cheju.ac.kr

An orange-coloured actinomycete, designated strain RP-B16^T, was isolated from a rhizosphere soil. Cells of strain RP-B16^T were strictly aerobic, Gram-positive-staining, oxidase-negative, catalase-positive, motile cocci. A phylogenetic tree, based on 16S rRNA gene sequences, showed that members of the genus Kineococcus were divided into two independent clusters and that strain RP-B16^T formed a coherent clade with the type strains of Kineococcus aurantiacus (99.5 % sequence similarity) and Kineococcus radiotolerans (99.1 %). Levels of 16S rRNA gene sequence similarity between strain RP-B16^T and Kineococcus marinus KST3-3^T and ‘Kineococcus tegulae’ T2A-S27 were lower than 97.0 %. Chemotaxonomic characteristics were typical of the genus Kineococcus in having meso-diaminopimelic acid as the diagnostic diamino acid, MK-9(H₂) as the predominant menaquinone, a polar lipid profile comprising diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and two unknown phospholipids, anteiso-C_{15 : 0} as the predominant fatty acid and a DNA G+C content of 73.8 mol%. Strain RP-B16^T showed levels of DNA–DNA relatedness of 34.6–37.3 % to Kineococcus aurantiacus DSM 7487^T and 31.2–32.7 % to Kineococcus radiotolerans DSM 14245^T. On the basis of phenotypic features and DNA–DNA hybridization data, strain RP-B16^T is considered to represent a novel species of the genus Kineococcus, for which the name Kineococcus rhizosphaerae sp. nov. is proposed. The type strain is RP-B16^T (=KCTC 19366^T =DSM 19711^T).