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Rhodoplanes pokkaliisoli sp. nov., a phototrophic alphaproteobacterium isolated from a waterlogged brackish paddy soil

¹ Bacterial Discovery Laboratory, Centre for Environment, Institute of Science and Technology, J. N. T. University, Kukatpally, Hyderabad 500 085, India
² Department of Plant Sciences, School of Life Sciences, University of Hyderabad, PO Central University, Hyderabad 500 046, India

Correspondence
Ch. Sasikala
sasi449{at}yahoo.ie or
r449{at}sify.com

A Gram-negative, rod-shaped, purple non-sulfur bacterial strain, designated JA415^T, was isolated from the mud of a pokkali rice field located on Vypeen Island, Ernakulam, Kerala, India. Strain JA415^T was motile by means of a single polar flagellum. Photo- and chemo-organoheterotrophic growth was observed using organic compounds as carbon sources and electron donors. Photo- and chemolithoautotrophic growth using thiosulfate as electron donor did not occur. Fermentative growth could not be demonstrated. Intracellular photosynthetic membranes were lamellar stacks parallel to the cytoplasmic membrane. Bacteriochlorophyll a and carotenoids of the spirilloxanthin series were present as photosynthetic pigments. Niacin, pantothenate and p-aminobenzoate were required as growth factors. C_{18 : 1}7c was the predominant cellular fatty acid component. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain JA415^T clustered with members of the genus Rhodoplanes in the class Alphaproteobacteria. On the basis of phenotypic and molecular genetic evidence, it is proposed that strain JA415^T be classified as a representative of a novel species of the genus Rhodoplanes, family Hyphomicrobiaceae, with the name Rhodoplanes pokkaliisoli sp. nov. The type strain is JA415^T (=KCTC 5711^T =NBRC 104972^T).

A phase-contrast micrograph, electron micrographs of ultrathin cell sections showing negatively stained cells with a single polar flagellum and the lamellar nature of the photosynthetic membranes and the whole-cell absorption spectrum of strain JA415^T are available as supplementary material with the online version of this paper.